Application of bovine microsatellite markers for genetic diversity analysis of Swiss yak (Poephagus grunniens)
Application of bovine microsatellite markers for genetic diversity analysis of Swiss yak (Poephagus grunniens)
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DOI：
10.1111/j.1365-2052.2005.01357.x
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发表时间：
2005-12-01
期刊：
影响因子：
2.4
通讯作者：
Stranzinger, G
中科院分区：
文献类型：
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作者：
Nguyen, TT;Genini, S;Stranzinger, G
为了评估牛微卫星标记在瑞士牦牛群体遗传学研究中的适用性，对10头牦牛的131个牛微卫星标记进行了测试。124个标记（94.6%）扩增出476个等位基因，其中117个标记（94.3%）具有多态性。每个位点的多态性标记之间的等位基因数从2到9不等。有7个位点（ILSTS 005、BMS 424 B、BMS 1825、BMS 672、BM 1314、ETH 123和BM 6017）未能扩增出牦牛基因组DNA。用两个牛Y染色体特异性微卫星标记（INRA 126和BM 861）扩增了公、母牦牛的基因组DNA。然而，牛Y染色体上的两个额外标记（INRA 124和INRA 189）仅从雄性扩增DNA。多态性标记。CaDBase提出的24个微卫星用于种内和跨种间比较，另外两个高度多态性标记（MHCII和TGLA 73）被用来研究瑞士牦牛群的遗传变异和种群结构，其中包括51头额外的动物。多态信息含量范围为0.355 - 0.752，而观察到的杂合度（H-0）范围为0.348 - 0.823。此外，一组13个标志物，组织成三个多重聚合酶链反应，进行了评价，用于常规亲子鉴定。这一组提供了一个排除概率在一个家庭的四个牦牛（父母和两个后代）的0.995。这些微卫星作为有用的工具，遗传特性的牦牛，这仍然是一个重要的家畜物种。
In order to assess the applicability of bovine microsatellite markers for population genetic studies in Swiss yak, 131 bovine microsatellite markers were tested on a panel of 10 animals. Efficient amplification was observed for 124 markers (94.6%) with a total of 476 alleles, of which 117 markers (94.3%) were polymorphic. The number of alleles per locus among the polymorphic markers ranged from two to nine. Seven loci (ILSTS005, BMS424B, BMS1825, BMS672, BM1314, ETH123 and BM6017) failed to amplify yak genomic DNA. Two cattle Y-chromosome specific microsatellite markers (INRA126 and BM861) amplified genomic DNA from both male and female yaks. However, two additional markers on cattle Y-chromosome (INRA124 and INRA189) amplified DNA from only males. Of the polymorphic markers. 24 microsatellites proposed by CaDBase for within- and cross-species comparisons and two additional highly polymorphic markers (MHCII and TGLA73) were used to investigate the genetic variability and the population structure of a Swiss yak herd that included 51 additional animals. The polymorphic information content ranged from 0.355 to 0.752, while observed heterozygosity (H-0) ranged from 0.348 to 0.823. Furthermore, a set of 13 markers, organized into three multiplex polymerase chain reactions, was evaluated for routine parentage testing. This set provided an exclusion probability in a family of four yaks (both parents and two offspring) of 0.995. These microsatellites serve as useful tools for genetic characterization of the yak, which continues to be an important domestic livestock species.