Verification of the introgression of Erianthus arundinaceus germplasm into sugarcane using molecular markers
Verification of the introgression of Erianthus arundinaceus germplasm into sugarcane using molecular markers
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DOI：
10.1111/j.1439-0523.2005.01099.x
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发表时间：
2005-08-01
期刊：
影响因子：
2
通讯作者：
McIntyre, CL
中科院分区：
文献类型：
--
作者：
Cai, Q;Aitken, K;McIntyre, CL
斑茅（Erianthus arundinaceus）具有宿根性好、耐旱、耐涝、抗病、生长势强等重要农艺性状，是甘蔗育种工作者感兴趣的亲本资源。然而，迄今为止，试图产生甘蔗（甘蔗属）之间的可育杂种。和E. arundinaceus一直不成功。微卫星标记，产生属特异性标记，并在甘蔗内高度多态性进行了鉴定。利用微卫星标记和5S rDNA PCR技术，对铁皮石斛（E. officinarum x E.）arundinaceus杂交，和两个甘蔗回交后代（BC 1）群体，其来源于所选的F-1克隆和甘蔗（甘蔗属）之间的杂交，以鉴定真正的假单胞菌属。BC 1后代。5SrDNAPCR标记和高度多态性的微卫星标记证实了这两个BC 1群体的F1亲本为蔗茅。officinarum x E.斑茅杂种，并允许从F-1亲本的自交系鉴定真正的BC 1后代。这是甘蔗属间杂种F1代BC 1后代的首次鉴定。用分子标记进行克隆，证实E.斑茅种质转化甘蔗。现在应该有可能从E.在甘蔗育种计划中。
Erianthus arundinaceus has a number of important agronomic traits including good ratooning ability, tolerance to both drought and water logging, disease resistance and vigour and is of interest as a potential source of parental germplasm to sugarcane breeders. However, to date, attempts to produce fertile hybrids between sugarcane (Saccharum spp.) and E. arundinaceus have been unsuccessful. Microsatellite markers that generated genus-specific markers and were highly polymorphic within sugarcane were identified. The microsatellite markers and 5S rDNA PCR were used to screen intergeneric (F-1) clones from Saccharum officinarum x E. arundinaceus crosses, and two Saccharum backcross progeny (BC1) populations derived from crosses between selected F-1 clones and sugarcane (Saccharum spp.), to identify genuine Saccharum spp. BC1 progeny. The 5S rDNA PCR marker and highly polymorphic microsatellites with Erianthus-specific bands confirmed the F-1 parent of the two putative BC1 populations was a S. officinarum x E. arundinaceus hybrid and allowed the identification of the genuine BC1 progeny from selfs of the F-1 parent. This is the first verification of BC1 progeny from an F-1 intergeneric hybrid x sugarcane (Saccharum spp.) clone with molecular markers and confirms the introgression of E. arundinaceus germplasm into sugarcane. It should now be possible to exploit genes of value from E. arundinaceus in sugarcane breeding programmes.