Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer
Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer
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DOI：
10.1023/a:1014471113511
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发表时间：
2002-01-01
期刊：
影响因子：
1.9
通讯作者：
Yang, TM
中科院分区：
文献类型：
--
作者：
Liu, ZY;Sun, QX;Yang, TM
小麦白粉病是由小麦白粉病菌（Erysiphe graminis f.）小麦赤霉病是世界上许多地区最重要的小麦病害之一。将来自以色列Rosh Pinna的野生二粒小麦（Triticum dicoccoides）登记号“C20”的抗白粉病基因通过杂交和回交成功地转移到六倍体小麦中。遗传分析表明，苗期白粉病抗性受一对显性基因控制。将杂交87-1/C20//2*8866的分离的BC 1F 2后代用于混合分离分析（BSA）。利用10-mer随机引物进行PCR扩增，在抗性和敏感DNA库之间产生多态性DNA片段。STS引物和小麦微卫星引物。Xgwm 159/430、Xgwm 159/460和Xgwm 159/500这3个标记与该抗性基因连锁。在两个分离群体中对多态性标记进行评价后，估计标记与抗病基因之间的距离为5- 6cM。利用中国春缺体-四体和双端体技术，将多态性标记和抗病基因定位在染色体5 BS臂上，并利用中国春缺失系将其物理定位在片段长度（FL）为0.41-0.43的基因富集区。由于在染色体臂5 BS上尚未发现抗白粉病基因，因此来源于C20的抗白粉病基因应为新基因，命名为Pm 30。
Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of the world. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum dicoccoides) accession 'C20', from Rosh Pinna, Israel, was successfully transferred to hexaploid wheat through crossing and backcrossing. Genetic analysis indicated that a single dominant gene controls the powdery mildew resistance at the seedling stage. Segregating BC1F2 progenies of the cross 87-1/C20//2*8866 were used for bulked segregant analysis (BSA). The PCR approach was used to generate polymorphic DNA fragments between the resistant and susceptible DNA pools by use of 10-mer random primers., STS primers, and wheat microsatellite primers. Three markers, Xgwm159/430, Xgwm159/460, and Xgwm159/500, were found to be linked to the resistance gene. After evaluating the polymorphic markers in two segregating populations, the distance between the markers and the mildew resistance gene was estimated to be 5-6 cM. By means of Chinese Spring nullisomic-tetrasomics and ditelosomics, the polymorphic markers and the resistance gene were assigned to chromosome arm 5BS and were physically mapped on the gene rich regions of fragment length (FL) 0.41-0.43 by Chinese Spring deletion lines. As no powdery mildew resistance gene has been reported on chromosome arm 5BS, the mildew resistance gene originating from C20 should be a new gene and is designated Pm30.