Focal perfusion of circulating cooled blood reduces the infarction volume and improves neurological outcome in middle cerebral artery occlusion
Focal perfusion of circulating cooled blood reduces the infarction volume and improves neurological outcome in middle cerebral artery occlusion
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DOI：
10.1179/174313209x443982
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发表时间：
2009-05-01
影响因子：
1.9
通讯作者：
Ling, Feng
中科院分区：
文献类型：
--
作者：
Cheng, Huakun;Ji, Xunming;Ling, Feng
目的：低温的神经保护作用已得到充分证实。采用全身低温治疗脑卒中的低温治疗受到降温速度的限制，且并发症严重。本研究的目的是确定通过输注进行局部脑冷却是否可以减少大脑中动脉（MCA）闭塞大鼠模型的梗塞体积并改善神经功能转归。方法：用中空丝阻断MCA 2小时，然后向缺血区域局部注入自循环冷动脉血（13-15℃）。这种冷血输注（< 0.6 毫升/分钟）在 5-10 分钟内将 MCA 供应区域的温度显着降低至 32-34 摄氏度。该低温过程维持30分钟。在评估第2天和第28天的神经学评分后，对所有动物实施安乐死，将它们的大脑切片并用苏木精和伊红(H&E)染色。计算其梗塞体积。结果：通过冷血输注的自循环引起脑部局部轻度低温，而直肠温度维持在正常范围内。与第 1 组（MCA 闭塞）和第 3 组（正常体温）相比，第 2 组（低体温）MCA 闭塞后梗塞体积显着缩小，神经功能结局显着改善（p < 0.05）。结论：自循环冷动脉血注入缺血组织引起的局部脑低温具有神经保护作用。 [神经研究2009； 31：340-345]
Objective: The neuroprotective effect of hypothermia has been well established. The use of hypothermia for the treatment of stroke by systemic hypothermia is limited by the cooling rate and has severe complications. The goal of this study was to determine if local cerebral cooling via infusion could reduce infarction volume and improve the neurological outcome in a rat model of middle cerebral artery (MCA) occlusion.Methods: A hollow filament was used to block the MCA for 2 hours, and then the ischemic territory was locally infused with autocirculating cold arterial blood (13-15 degrees C). This cold blood infusion (< 0.6 ml/min) significantly reduced the temperature of the MCA supplied territory to 32-34 degrees C in 5-10 minutes. This hypothermic procedure was maintained for 30 minutes. After evaluating the neurological score at 2 days and 28 days, all animals were euthanized and their brains were sectioned and stained with hematoxylin and eosin (H&E). Their infarct volumes were calculated.Results: Local mild hypothermia in the brain was induced by autocirculation with a cold blood infusion, whereas the rectal temperature was maintained within the normal range. The infarction volume was significantly reduced and the neurological outcome was significantly improved (p < 0.05) after MCA occlusion in Group 2 (hypothermia) compared with Group 1 (MCA occlusion) and Group 3 (normothermia).Conclusion: Local brain hypothermia induced by autocirculating cold arterial blood infused into ischemic tissue has neuroprotective effects. [Neurol Res 2009; 31: 340-345]