Time course of inflammation, oxidative stress and tissue damage induced by hyperoxia in mouse lungs
Time course of inflammation, oxidative stress and tissue damage induced by hyperoxia in mouse lungs
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DOI：
10.1111/j.1365-2613.2012.00823.x
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发表时间：
2012-08-01
影响因子：
3
通讯作者：
Valenca, Samuel S.
中科院分区：
文献类型：
--
作者：
Nagato, Akinori C.;Bezerra, Frank S.;Valenca, Samuel S.
反刍家畜的中枢神经系统（CNS）感染，如李斯特菌病，是兽医和公共卫生的主要关注点。迄今为止，还没有针对反刍动物中枢神经系统感染的宿主特异性体外模型。在此，我们建立并评估了反刍动物器官型脑切片作为体外模型的适用性，以研究单核细胞增生李斯特菌CNS感染的机制。反刍动物经常受到致命的李斯特菱形脑炎的影响，这种疾病与人类的情况非常相似。因此，不仅从兽医的角度，而且从公共卫生的角度来看，更好地了解反刍动物中宿主病原体的相互作用是有意义的。在屠宰场获得大脑，海马体和小脑切片培养49天。每周评估细胞活力和细胞群组成。在体外观察存活神经元、星形胶质细胞、小胶质细胞和少突胶质细胞长达49天。切片培养物感染单核增生乳杆菌，并监测感染动力学。采用双免疫荧光法对感染的脑细胞进行鉴定，并与自然病例进行比较。与自然感染相似，受感染的脑切片显示单核增生乳杆菌和细菌的局灶性复制主要在小胶质细胞中观察到，但也在星形胶质细胞中观察到，并与轴突相关。这些结果表明，器官型牛脑切片培养物存活时间较长，并且容易被单核细胞增生乳杆菌感染。因此，它们是研究李斯特脑炎和其他神经感染性疾病中宿主-病原体相互作用方面的合适模型。
Central nervous system (CNS) infections in ruminant livestock, such as listeriosis, are of major concern for veterinary and public health. To date, no host-specific in vitro models for ruminant CNS infections are available. Here, we established and evaluated the suitability of organotypic brain-slices of ruminant origin as in vitro model to study mechanisms of Listeria monocytogenes CNS infection. Ruminants are frequently affected by fatal listeric rhombencephalitis that closely resembles the same condition occurring in humans. Better insight into hostpathogen interactions in ruminants is therefore of interest, not only from a veterinary but also from a public health perspective. Brains were obtained at the slaughterhouse, and hippocampal and cerebellar brain-slices were cultured up to 49 days. Viability as well as the composition of cell populations was assessed weekly. Viable neurons, astrocytes, microglia and oligodendrocytes were observed up to 49 days in vitro. Slice cultures were infected with L. monocytogenes, and infection kinetics were monitored. Infected brain cells were identified by double immunofluorescence, and results were compared to natural cases of listeric rhombencephalitis. Similar to the natural infection, infected brain-slices showed focal replication of L. monocytogenes and bacteria were predominantly observed in microglia, but also in astrocytes, and associated with axons. These results demonstrate that organotypic brain-slice cultures of bovine origin survive for extended periods and can be infected easily with L. monocytogenes. Therefore, they are a suitable model to study aspects of hostpathogen interaction in listeric encephalitis and potentially in other neuroinfectious diseases.