Increased heme oxygenase activity in splanchnic organs from portal hypertensive rats: role in modulating mesenteric vascular reactivity
Increased heme oxygenase activity in splanchnic organs from portal hypertensive rats: role in modulating mesenteric vascular reactivity
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DOI：
10.1016/s0168-8278(01)00010-1
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发表时间：
2001-06-01
影响因子：
25.7
通讯作者：
Bonkovsky, HL
中科院分区：
文献类型：
--
作者：
Fernandez, M;Lambrecht, RW;Bonkovsky, HL
背景/目的：我们最近发现血红素氧合酶-1在门脉高压大鼠内脏器官中表达上调。在本研究中，我们评估是否血红素氧合酶的酶活性增加，门静脉高压症大鼠内脏器官，血红素氧合酶和一氧化氮合酶的相对贡献的血管低反应性门脉高压症。方法：血红素氧合酶活性测定门静脉高压症和假手术大鼠内脏器官。血红素氧合酶和一氧化氮合酶抑制对氯化钾和甲氧胺的压力反应的影响进行了评估，在灌注肠系膜血管床门脉高压症和假手术rats.Results：血红素氧合酶活性增加，肠系膜，肠，肝，脾的门脉高压症大鼠。门脉高压大鼠对氯化钾的低反应性在同时抑制血红素氧合酶和一氧化氮合酶后被克服，但仅在单独抑制一氧化氮合酶后部分减弱。结论：门脉高压大鼠内脏器官血红素氧合酶活性增高，提示血红素氧合酶参与门脉高压大鼠内脏器官对氯化钾的低反应性，而不是对甲氧胺的低反应性。(C)2001年欧洲肝脏研究协会。由Elsevier Science B，V出版，保留所有权利。
Background/Aims: We have recently demonstrated that heme oxygenase-1 is upregulated in splanchnic organs of portal hypertensive rats. In the present study, we assessed whether heme oxygenase enzymatic activity is increased in splanchnic organs of portal hypertensive rats, and the relative contribution of heme oxygenase and nitric oxide synthase to the vascular hyporeactivity in portal hypertension.Methods: Heme oxygenase activity was measured in splanchnic organs of portal hypertensive and sham-operated rats. The effects of heme oxygenase and nitric oxide synthase inhibition on pressure responses to potassium chloride and methoxamine were assessed in perfused mesenteric vascular beds of portal hypertensive and sham-operated rats.Results: Heme oxygenase activity was increased in the mesentery, intestine, liver, and spleen of portal hypertensive rats. The hyporeactivity to potassium chloride in portal hypertensive rats was overcome after simultaneous inhibition of both heme oxygenase and nitric oxide synthase, but only partially attenuated after nitric oxide synthase inhibition alone. The hyporeactivity to methoxamine was completely reversed after nitric oxide synthase blockade.Conclusions: These results demonstrate that heme oxygenase activity is increased in splanchnic organs of portal hypertensive rats, They also suggest that heme oxygenase contributes to the hyporeactivity to potassium chloride, but not to methoxamine, in portal hypertensive rats. (C) 2001 European Association for the Study of the Liver. Published by Elsevier Science B,V, All rights reserved.