Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo.
Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo.
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体内果蝇异质核核糖核蛋白过度表达导致外显子跳跃。
DOI：
10.1073/pnas.92.6.1822
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发表时间：
1995
影响因子：
11.1
通讯作者：
Hirsh,J
中科院分区：
文献类型：
--
作者：
Shen,J;Zu,K;Cass,CL;Beyer,AL;Hirsh,J
异质核核糖核蛋白 (hnRNP) 是丰富的 RNA 结合蛋白，参与剪接调节。在这里，我们研究了果蝇 hnRNP 在活体动物剪接调节中的作用。我们发现果蝇 hnRNP HRB98DE 的过度表达会导致体内果蝇多巴脱羧酶 (Ddc) 前体 mRNA 中所有内部外显子的跳跃。这些结果表明HRB98DE具有促进末端剪接位点的使用的剪接活性。即使高水平的 HRB98DE 持续至少 24 小时，过量的 HRB98DE 对 Ddc 剪接的影响也是暂时的。这表明果蝇幼虫可以诱导补偿机制来抵消过量 HRB98DE 的影响。
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are abundant RNA-binding proteins that are implicated in splicing regulation. Here we investigate the role of a Drosophila hnRNP in splicing regulation in living animals. We find that overexpression of the Drosophila hnRNP HRB98DE leads to skipping of all internal exons in the Drosophila dopa decarboxylase (Ddc) pre-mRNA in vivo. These results indicate that HRB98DE has a splicing activity that promotes use of terminal splice sites. The effect of excess HRB98DE on Ddc splicing is transient, even though high levels of HRB98DE persist for at least 24 hr. This suggests that Drosophila larvae can induce a compensating mechanism to counteract the effects of excess HRB98DE.