Phytochrome A and B Function Antagonistically to Regulate Cold Tolerance via Abscisic Acid-Dependent Jasmonate Signaling
Phytochrome A and B Function Antagonistically to Regulate Cold Tolerance via Abscisic Acid-Dependent Jasmonate Signaling
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光敏色素 A 和 B 通过脱落酸依赖性茉莉酸信号传导拮抗地调节耐寒性
DOI：
10.1104/pp.15.01171
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发表时间：
2016-01-01
期刊：
影响因子：
7.4
通讯作者：
Zhou, Yanhong
中科院分区：
文献类型：
--
作者：
Wang, Feng;Guo, Zhixin;Zhou, Yanhong
光信号和植物激素都影响植物的生长、发育和胁迫反应；然而，这两种信号通路在应对寒冷时的交互作用仍未得到充分研究。本文报道了光敏色素A （phyya）和phyB感知的远红光（FR）和红光(R)分别对番茄（Solanum lycopersicum）的耐寒性产生正向和负向调节，这与植物激素如脱落酸（ABA）和茉莉酸（JA）的水平以及ABA和JA相关基因和C-REPEAT结合因子（CBF）胁迫信号通路基因的转录水平有关。R/FR比值的降低没有改变phyA突变体的耐寒性、ABA和JA积累以及ABA和JA相关基因和CBF通路基因的转录水平；而在野生型和phyB植物中，随着R/FR比的降低，这些都显著增加。尽管低R/FR处理并没有赋予ABA缺乏（notabilis [not]）和JA缺乏（prosystem -mediated responses2 [spr2]）突变体耐寒性，但它上调了spr2突变体中ABA的积累和信号传导，而对非突变体中的JA水平和信号传导没有影响。叶面施用ABA和JA进一步证实，JA在ABA下游激活CBF通路，介导光质量介导的抗寒性。综上所述，phyA和phyB拮抗调节番茄抗寒性，主要涉及FR光诱导的phyA激活诱导ABA信号和随后的JA信号，导致CBF通路的激活和番茄植物的冷反应。
Light signaling and phytohormones both influence plant growth, development, and stress responses; however, cross talk between these two signaling pathways in response to cold remains underexplored. Here, we report that far-red light (FR) and red light (R) perceived by phytochrome A (phyA) and phyB positively and negatively regulated cold tolerance, respectively, in tomato (Solanum lycopersicum), which were associated with the regulation of levels of phytohormones such as abscisic acid (ABA) and jasmonic acid (JA) and transcript levels of ABA- and JA-related genes and the C-REPEAT BINDING FACTOR (CBF) stress signaling pathway genes. A reduction in the R/FR ratio did not alter cold tolerance, ABA and JA accumulation, and transcript levels of ABA-and JA-related genes and the CBF pathway genes in phyA mutant plants; however, those were significantly increased in wild-type and phyB plants with the reduction in the R/FR ratio. Even though low R/FR treatments did not confer cold tolerance in ABA-deficient (notabilis [not]) and JA-deficient (prosystemin-mediated responses2 [spr2]) mutants, it up-regulated ABA accumulation and signaling in the spr2 mutant, with no effect on JA levels and signaling in the not mutant. Foliar application of ABA and JA further confirmed that JA functioned downstream of ABA to activate the CBF pathway in light quality-mediated cold tolerance. It is concluded that phyA and phyB function antagonistically to regulate cold tolerance that essentially involves FR light-induced activation of phyA to induce ABA signaling and, subsequently, JA signaling, leading to an activation of the CBF pathway and a cold response in tomato plants.