A L-[3H]glutamate binding site on glia: an autoradiographic study on implanted astrocytes.
A L-[3H]glutamate binding site on glia: an autoradiographic study on implanted astrocytes.
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胶质细胞上的 L-[3H]谷氨酸结合位点：植入星形胶质细胞的放射自显影研究。
DOI：
10.1016/0006-8993(87)90281-2
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Cotman,CW
中科院分区：
文献类型：
--
作者：
Bridges,RJ;Kesslak,JP;Nieto-Sampedro,M;Broderick,JT;Yu,J;Cotman,CW
在本研究中，将培养的星形胶质细胞植入大鼠下丘，以创建一个星形胶质细胞富集的领域，可以进行放射自显影检查。用抗胶质细胞酸性蛋白（GFA）免疫细胞化学证实星形胶质细胞的存在。我们报告存在一个氯离子依赖性谷氨酸结合位点上植入的星形胶质细胞。在氯化物的存在下，在植入区域中检测到的特异性谷氨酸结合是在相应的对侧区域中发现的特异性谷氨酸结合的5倍。当氯化物被乙酸盐取代时，谷氨酸与星形胶质细胞的结合减少了80%以上。氯离子依赖性结合对红藻氨酸（KA）和N-甲基-d-天冬氨酸（NMDA）的抑制不敏感，对使君子酸、l-天冬氨酸、l-2-氨基-4-膦酰基丁酸和l-α-氨基己二酸敏感。的药理学的结合是非常相似的，在体外谷氨酸结合到膜从培养的星形胶质细胞和在胶质瘤细胞系中确定的氯依赖性运输系统。我们的结论是谷氨酸与星形胶质细胞的相互作用是一个重要的组成部分，在脑切片中观察到的总谷氨酸结合。
In the present study cultured astrocytes were implanted into the inferior colliculus of rats to create an astrocyte-enriched field that could be examined autoradiographically. The presence of the astrocytes was confirmed with anti-glial fibrillary acidic protein (GFA) immunocytochemistry. We report the presence of a chloride-dependent glutamate binding site on the implanted astrocytes. In the presence of chloride, the specific glutamate binding detected in the implant area was 5-fold greater than that found in a corresponding contralateral region. When the chloride was replaced with acetate, glutamate binding to the astrocytes decreased by more than 80%. The chloride-dependent binding to the astrocytes was insensitive to inhibition by kainic acid (KA) and N-methyl-d-aspartate (NMDA) and sensitive to quisqualate,l-aspartate,l-2-amino-4-phosphonobutyrate, andl-α-aminoadipate. The pharmacology of the binding was very similar to that of the in vitro glutamate binding to membranes from cultured astrocytes and to that of a chloride-dependent transport system identified in a glioma cell line. We conclude that the interaction of glutamate with astrocytes is an important component of the total glutamate binding observed in brain slices.