PARASITIC CASTRATION OF Plutella xylostella LARVAE INDUCED BY POLYDNAVIRUSES AND VENOM OF Cotesia vestalis AND Diadegma semiclausum
PARASITIC CASTRATION OF Plutella xylostella LARVAE INDUCED BY POLYDNAVIRUSES AND VENOM OF Cotesia vestalis AND Diadegma semiclausum
复制标题
多DNA病毒和小菜蛾、半角菜蛾毒对小菜蛾幼虫的寄生去势
DOI：
10.1002/arch.20279
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发表时间：
2009-01-01
影响因子：
2.2
通讯作者：
Chen, Xue-xin
中科院分区：
文献类型：
--
作者：
Doi, Su-fen;Cai, Dong-zhang;Chen, Xue-xin
In the present study, we used gamma-ray to irradiate the female parasitoids to make wasp eggs infertile, resulting in pseudoparasitization, which allowed the analysis of maternal secretions such as polydnaviruses (PDVs) and venom in the absence of larval secretions or teratocytes by the growing parasitoids. We then investigated the spermatogenesis and components of testicular proteins of male Plutella xylostella larvae pseudoparasitized by two endoparasitoids (Cotesia vestalis and Diadegma semiclausum). The results showed that pseudoparasitims by the two endoparasitoids at the early third instar host larvae both induced smaller testes in size than those of nonparasitized host larvae. Both of them caused parasitic castration, and the degree of castration is almost as severe as in naturally parasitized hosts. This suggested that PDVs and venom played a major role in the degeneration of host testes. There are significant differneces in the degree of castration induced by the two endoparasitoids, with respect to testicular growth, testicular protein concentrations, and histological changes of germ cells. Cotesia vestalis bracovirus always has a significantly stronger effect on host testicular growth and developmental than D. semiclausum ichnovirus. SDS-PAGE analysis indicated that synthesis of P 65 and P 67 proteins were clearly inhibited in testes of hosts that were pseudoparasitized by C. vestalis while reduction in synthesis of other proteins was not evident. (C) 2008 Wiley Periodicals, Inc.
