UCST-type phase transition driven by protein-derived polypeptide employing gelatin and chitosan.
UCST-type phase transition driven by protein-derived polypeptide employing gelatin and chitosan.
复制标题
由使用明胶和壳聚糖的蛋白质衍生多肽驱动的 UCST 型相变。
DOI：
10.1002/pat.4033
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Matsukuma;D.;Sambai;T.;and Otsuka;H.
在这里，我们描述了由明胶和壳聚糖组成的聚电解质络合物(G/C络合物)的温敏可逆相变行为。G/C络合物的水分散体在30°C附近有明显的上临界溶解温度(UCST)。基于分子内和分子间相互作用的变化，热敏相变行为表现出良好的可逆性和较大的热滞。用圆二色谱分析了G/C复合体的UCST与明胶的螺旋熔融温度之间的高度相关性，表明G/C复合体的相变与明胶的二级结构(螺旋-螺旋)转变相对应。值得注意的是，在尿素存在下，G/C复合体的UCST移动到较低的温度，这是众所周知的破坏明胶稳定的因素，而盐的加入则导致G/C复合体的溶解。预计这项研究的结果将对UCST类型热敏材料的制备产生重大影响，这种材料可以在水生理条件下使用众所周知的生物聚合物。这种蛋白质衍生的功能材料响应了二级结构的转变，也可以用于开发新型UCST类型的热敏生物材料。版权所有©2017 John Wiley&Sons，Ltd.
Here, we describe the thermosensitive reversible phase transition behaviors of polyelectrolyte complex composed of gelatin and chitosan (G/C complex). An aqueous dispersion of the G/C complexes showed a clear upper critical solution temperature (UCST) at around 30°C. The thermosensitive phase transition behavior showed excellent reversibility and large thermal hysteresis as a usual phenomenon based on the intra‐ and inter‐molecular interaction change. A high correlation was observed between the UCST of the G/C complex and the helix‐melting temperature of gelatin by circular dichroism, which suggested that the phase transition of the G/C complex corresponded to the secondary structure (helix‐coil) transition of gelatin. Notably, the UCST of the G/C complex shifted to lower temperatures in the presence of urea, which is well known to destabilize gelatin, whereas the addition of salt led to the dissolution of the G/C complex. It is envisaged that the results of this study will have a significant impact on the fabrication of UCST‐type thermosensitive materials, which can be utilized under aqueous physiological conditions using well‐known biopolymers. This protein‐derived functional material, which responds to the secondary structure transition, could also be used for the development of novel UCST‐type thermosensitive biomaterials. Copyright © 2017 John Wiley & Sons, Ltd.