Synchronization of Yeast
Synchronization of Yeast
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DOI：
10.1007/978-1-4939-6603-5_14
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Schneider, Brandt L.
中科院分区：
文献类型：
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作者：
Smith, Jessica;Manukyan, Arkadi;Schneider, Brandt L.
芽殖酵母酿酒酵母和裂殖酵母粟酒裂殖酵母是研究细胞周期控制遗传学的最简单和最强大的模型系统之一。由于酵母在简单经济的培养基中生长非常迅速，因此可以很容易地获得大量细胞用于细胞周期的遗传、分子和生物化学研究。同步培养的使用大大有助于细胞周期研究的简便和解释。原则上，有两种获得同步化酵母群体的一般方法。阻断和释放方法可用于诱导细胞周期同步。或者，离心淘洗可用于选择同步群体。由于每种方法都有其固有的优点和缺点，因此使用多种方法有助于推广结果。概述了最常用的方法来产生同步酵母培养物是沿着工作说明：一个部分，其中包括实际的意见，实验考虑和观察，并提示有关的利弊固有的每种方法。
The budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces pombe are amongst the simplest and most powerful model systems for studying the genetics of cell cycle control. Because yeast grows very rapidly in a simple and economical media, large numbers of cells can easily be obtained for genetic, molecular, and biochemical studies of the cell cycle. The use of synchronized cultures greatly aids in the ease and interpretation of cell cycle studies. In principle, there are two general methods for obtaining synchronized yeast populations. Block-and-release methods can be used to induce cell cycle synchrony. Alternatively, centrifugal elutriation can be used to select synchronous populations. Because each method has innate advantages and disadvantages, the use of multiple approaches helps in generalizing results. An overview of the most commonly used methods to generate synchronized yeast cultures is presented along with working Notes : a section that includes practical comments, experimental considerations and observations, and hints regarding the pros and cons innate to each approach.