A new quinoline-derived highly-sensitive fluorescent probe for the detection, of hydrazine with excellent large-emission-shift ratiometric response
A new quinoline-derived highly-sensitive fluorescent probe for the detection, of hydrazine with excellent large-emission-shift ratiometric response
复制标题
一种新型喹啉衍生的高灵敏度荧光探针，用于检测肼，具有优异的大发射位移比率响应
DOI：
10.1016/j.talanta.2018.12.015
复制
发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Stadler Florian J
中科院分区：
文献类型：
--
作者：
Wu Qingqing;Zheng Jianlu;Zhang Wencui;Wang Jianbo;Liang Wenlang;Stadler Florian J
联氨是一种重要的工业原料，但在环境中过度暴露，毒性极大，对人体健康危害极大，因此监测联氨具有重要意义。本文合成了一种基于羟基-苯并[d]恶唑取代喹啉结构的新型高荧光荧光分子rebq - oh，并与4-生物丁酸酯化，得到了一种选择性检测联氨的荧光探针bqab。探针遵循SN2（亲核取代）-环化传感机制，具有显著的响应性、良好的灵敏度和选择性。在水溶液中的光谱实验表明，bqabreb对肼具有良好的比例荧光响应，在感应反应前后有两个分离良好的发射带。发射峰从387 nm位移到521 nm，位移超过130 nm，实现了超过3000倍的显著信号增强。由于光谱响应大，获得了5.8 nM （0.19 ppb）的极低检出限。选择性实验表明，bqabrb对其他可能干扰的分析物几乎没有光谱响应。制备了探针涂布试纸，并成功地应用于气态联氨的检测。此外，在环境样品和生物样品（活细胞）中检测联氨的潜在应用已被证明。
Hydrazine is an important industrial material yet highly toxic and extremely harmful to people's health when over-exposed in the environment, thus monitoring hydrazine is of great significance. In this work, a novel highly fluorescent fluorophoreBQ-OH, based on hydroxyl- and benzo[d]oxazole-substituted quinoline structure, was synthesized and esterified with 4-biomobutyric acid to afford a fluorescent probeBQABrfor the selective detection of hydrazine. The probe follows SN2(nucleophilic substitution)-cyclization sensing mechanism with remarkable response, excellent sensitivity and selectivity. Spectra experiments in aqueous solutions demonstrated thatBQABrexhibited an excellent ratiometric fluorescence response toward hydrazine with two well separated emission bands before/after sensing reaction. Emission peak shifted over 130 nm from 387 nm to 521 nm, and unexpectedly outstanding ratio signal enhancement over 3000-fold was achieved. Due to the large spectra response, a very low detection limit of 5.8 nM (0.19 ppb) was obtained. Selectivity experiment was performed, showingBQABrhad nearly no spectra response to other possible disturbing analytes. The probe-coated test papers were fabricated and successfully applied to detect gaseous hydrazine. Furthermore, potential application for the detection of hydrazine in both environmental samples and biological samples (living cells) has been demonstrated.