Toll-like receptor 2 is at least partly involved in the antitumor activity of glycoprotein from Chlorella vulgaris
Toll-like receptor 2 is at least partly involved in the antitumor activity of glycoprotein from Chlorella vulgaris
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DOI：
10.1016/s1567-5769(02)00002-4
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发表时间：
2002-03-01
影响因子：
5.6
通讯作者：
Yoshikai, Y
中科院分区：
文献类型：
--
作者：
Hasegawa, T;Matsuguchi, T;Yoshikai, Y
Toll样受体（TLR）通过识别各种细菌成分参与先天免疫。我们以前曾报道，从小球藻培养基中纯化的ARS-2的活性物质是一种分子量为63，100 amu的糖蛋白，并且该糖蛋白具有抗肿瘤活性，ARS-2中的蛋白质部分是这种抗肿瘤活性所必需的。在这里，我们表明，ARS-2刺激脾贴壁细胞从C3 H/HeJ缺乏功能性TLR 4产生白细胞介素-12（IL-12）p40，而这种细胞因子的产生显着受损的ARS-2刺激的脾贴壁细胞从TLR 2基因敲除小鼠。小鼠TLR 2（mTLR 2）和小鼠CD 14（mCD 14）的过表达赋予ARS-2对人HEK 293细胞的核因子-κ B活化的诱导作用。这些结果表明，TLR 2信号至少部分参与了来自C.普通的(C)2002 Elsevier Science B. V.保留所有权利。
Toll-like receptors (TLR) are involved in innate immunity by recognizing various bacterial components. We have previously reported that an active substance of ARS-2 purified from the culture medium of Chlorella vulgaris was a glycoprotein with a molecular weight of 63,100 amu and that this glycoprotein expressed antitumor activity, with the protein moiety in ARS-2 being necessary for this antitumor activity. Here, we show that ARS-2 stimulated spleen-adherent cells from C3H/HeJ lacking functional TLR4 to produce interleukin-12 (IL-12) p40, whereas such cytokine production was significantly impaired in ARS2-stimulated spleen-adherent cells from TLR2 knockout mice. The overexpression of mouse TLR2 (mTLR2) and mouse CD14 (mCD14) conferred the ARS-2 inducibility of nuclear factor-kappaB activation to human HEK 293 cells. These results suggest that TLR2 signaling is at least partly involved in the antitumor activity of the water-soluble antitumor glycoprotein from C. vulgaris. (C) 2002 Elsevier Science B.V. All rights reserved.