Morphine promotes simian acquired immunodeficiency syndrome virus replication in monkey peripheral mononuclear cells: induction of CC chemokine receptor 5 expression for virus entry.
Morphine promotes simian acquired immunodeficiency syndrome virus replication in monkey peripheral mononuclear cells: induction of CC chemokine receptor 5 expression for virus entry.
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吗啡促进猴外周单核细胞中猴获得性免疫缺陷综合征病毒复制：诱导病毒进入的 CC 趋化因子受体 5 表达。
DOI：
10.1086/340816
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Chuang,RonaldY
中科院分区：
文献类型：
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作者：
Suzuki,Shunji;Chuang,AnnJ;Chuang,LindaF;Doi,RoyH;Chuang,RonaldY
长期服用阿片类药物的恒河猴比未暴露于阿片类药物的恒河猴更容易感染猴免疫缺陷病毒（SIV）mac 239株（SIVmac 239）。吗啡依赖性猴子血浆病毒血症的增加使得无需与组织培养细胞系共培养即可在动物外周血单核细胞（PBMC）中检测到SIV。相比之下，在没有共培养的情况下，来自吗啡初始SIVmac 239感染动物的PBMC的病毒滴度是检测不到的。从非感染动物中分离的PBMC在体外用硫酸吗啡处理产生β-趋化因子受体5（CCR 5）表达的增加。由于SIVmac 239和人类免疫缺陷病毒（HIV）都需要CCR 5进入细胞，吗啡在促进SIV感染中的独特作用可能提供了一种机制，以解释吸毒人群中HIV疾病的高发病率。
Rhesus monkeys {it(Macaca mulatta)} chronically administered opioids were more susceptible to simian immunodeficiency virus (SIV) strain mac239 (SIVmac239) infection than those without prior exposure to opioids. Increased plasma viremia in morphine-dependent monkeys allowed SIV to be detected in the animals' peripheral blood mononuclear cells (PBMC) without cocultivation with a tissue culture cell line. In contrast, virus titers from the PBMC of morphine-naive SIVmac239-infected animals were undetectable in the absence of cocultivation. PBMC isolated from noninfected animals and treated with morphine sulfate in vitro produced an increase in the expression of β-chemokine receptor 5 (CCR5). Because both SIVmac239 and human immunodeficiency virus (HIV) require CCR5 for cell entry, the unique role of morphine in promoting SIV infection may provide a mechanism to account for the high incidence of HIV disease among drug-using populations.