GATA2 rs2335052 Polymorphism Predicts the Survival of Patients with Colorectal Cancer.
GATA2 rs2335052 Polymorphism Predicts the Survival of Patients with Colorectal Cancer.
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DOI：
10.1371/journal.pone.0136020
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Su X
中科院分区：
文献类型：
--
作者：
Liu X;Jiang B;Wang A;Di J;Wang Z;Chen L;Su X
加塔结合蛋白2（GATA 2）是一种转录因子，在血液恶性肿瘤和各种实体瘤的进展中具有重要作用。我们的前期研究表明GATA 2高表达与结直肠癌复发相关。然而，GATA 2单核苷酸多态性（SNPs）对结直肠癌生存率的影响仍不清楚。我们在PCR扩增后使用桑格测序对GATA 2 SNP rs 2335052进行基因分型，并通过免疫组化测定180例CRC患者队列中GATA 2的表达。采用Kaplan-Meier生存分析和考克斯比例风险回归分析GATA 2 rs 2335052基因型与结直肠癌临床预后的关系。我们发现rs 2335052基因型与GATA 2的表达之间没有显著相关性。Kaplan-Meier生存分析显示，与携带rs 2335052 GG变异基因型的患者相比，携带rs 2335052 A等位基因的患者复发风险增加，无病生存率降低（P = 0.021）。单因素和多因素考克斯回归分析显示GATA 2 SNP rs 2335052是CRC患者DFS的独立危险因素。我们的研究结果表明，GATA 2 SNP rs 2335052是CRC患者预后的独立预测因子。这提高了SNP rs 2335052可能作为预测根治性结肠切除术后CRC复发的潜在指标的可能性。
GATA binding protein 2 (GATA2) is a transcription factor that has essential roles in hematologic malignancies and progression of various solid tumors. Our previous studies suggested that high GATA2 expression is associated with recurrence of colorectal cancer (CRC). However, the influence of GATA2 single nucleotide polymorphisms (SNPs) on the survival of CRC remains unknown. We genotyped GATA2 SNP rs2335052 using Sanger sequencing after PCR amplification, and determined GATA2 expression by immunohistochemistry in a cohort of 180 CRC patients. Kaplan-Meier survival analysis and Cox proportional hazard regression were used to analyze the association between the GATA2 rs2335052 genotypes and the clinical outcome of CRC. We found that there was no significant correlation between the rs2335052 genotypes and the expression of GATA2. However, the Kaplan-Meier survival analysis suggested that the carriers of the A-allele of SNP rs2335052 were significantly associated with increased risk of recurrence and reduced disease-free survival (DFS), compared with those carrying the variant genotype of GG in rs2335052 (P = 0.021). Moreover, univariate and multivariate Cox regression analyses revealed that GATA2 SNP rs2335052 was an independent risk factor for the DFS of CRC patients. Our results demonstrated that GATA2 SNP rs2335052 is an independent predictor for prognosis of CRC patients. This raised the possibility that SNP rs2335052 may serve as a potential indicator for predicting recurrence of CRC after curative colectomy.