IMMUNOLOGICAL MEMORY AFTER IMMUNIZATION WITH ORAL CHOLERA B-SUBUNIT WHOLE-CELL VACCINE IN SWEDISH VOLUNTEERS
IMMUNOLOGICAL MEMORY AFTER IMMUNIZATION WITH ORAL CHOLERA B-SUBUNIT WHOLE-CELL VACCINE IN SWEDISH VOLUNTEERS
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DOI：
10.1016/0264-410x(94)90176-7
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发表时间：
1994-09-01
期刊：
影响因子：
5.5
通讯作者：
HOLMGREN, J
中科院分区：
文献类型：
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作者：
JERTBORN, M;SVENNERHOLM, AM;HOLMGREN, J
在瑞典志愿者中，通过检测初次免疫后10个月给予单剂量B亚单位-全细胞（B-WC）霍乱疫苗的免疫应答，研究了口服两剂或三剂B-WC霍乱疫苗诱导免疫记忆的能力。研究了血清抗体应答和外周血抗体分泌细胞（ASC）应答。因为这些应答似乎反映了用B-WC疫苗口服免疫后的肠粘膜伊加免疫应答。先前免疫的志愿者对单剂量B-WC疫苗的应答频率更高，血清中伊加和IgG抗毒素抗体以及杀弧菌抗体水平也高于先前未接种疫苗的对照组。ISA-ASC对霍乱毒素B亚单位的反应在致敏志愿者中也高于对照组。两剂B-WC疫苗诱导霍乱免疫记忆的效果与三剂相当。以重组B亚单位为基础构建的B-WC霍乱疫苗具有与第一代B-WC疫苗相同的免疫记忆能力。
The capacity of peroral immunization with either two or three doses of B subunit-whole cell (B-WC) cholera vaccine to induce immunological memory was examined in Swedish volunteers by testing the immune responses to a single dose of B-WC vaccine given 10 months after the initial immunization, Antibody responses in serum and antibody-secreting cell (ASC) responses in peripheral blood were studied, since these responses seem to reflect the gut mucosal IgA immune responses after oral immunization with B-WC vaccine. Previously immunized volunteers responded to a single dose of B-WC vaccine more frequently and with higher levels of IgA and IgG antitoxin antibodies as well as vibriocidal antibodies in serum than did previously unvaccinated controls. The ISA-ASC responses to cholera toxin B subunit were also higher in primed volunteers than in controls. Two doses of B-WC vaccine were as effective as three doses in inducing immunological memory for cholera immunity. A new B-WC cholera vaccine based on recombinant B subunit had the same capacity as the first generation of B-WC vaccine to induce immunological memory for cholera antitoxin immunity.