Degradation of chlorobenzenes by a strain of Acidovorax avenae isolated from a polluted aquifer
Degradation of chlorobenzenes by a strain of Acidovorax avenae isolated from a polluted aquifer
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DOI：
10.1016/j.chemosphere.2005.03.003
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发表时间：
2005-09-01
期刊：
影响因子：
8.8
通讯作者：
Wunderlin, DA
中科院分区：
文献类型：
--
作者：
Monferrán, MV;Echenique, JR;Wunderlin, DA
从苏基亚河（科尔多瓦，阿根廷）的污染场地，在有氧条件下使用1，2-二氯苯（1，2-DCB）作为唯一的碳源，在15天内驯化的地下微生物群落分离。从这个驯化的社区，我们分离和鉴定的16 S rDNA分析，这是能够进行完全生物降解的1，2-DCB在两天内提供化学计量的氯化物的燕麦噬酸菌菌株。该纯菌株还进行了氯苯（CB）、1，3-DCB和1，4-DCB的生物降解试验，结果与使用1，2-DCB的实验结果相似。通过PCR扩增和DNA测序，检测到编码氯苯双加氧酶大亚基催化位点的芳香环羟基化双加氧酶（ARHDO）α亚基基因核心。这些结果表明，A.麦长管蚜可以使用分解代谢途径，通过ARHDO系统，导致在生物降解的第一步形成氯代儿茶素，进一步的氯化物释放和随后的路径，显示完全底物消耗。(C)2005爱思唯尔有限公司保留所有权利。
A subsurface microbial community was isolated from a polluted site of Suquia River (Cordoba-Argentina), acclimated during 15 days in aerobic conditions using 1,2-dichlorobenzene (1,2-DCB) as the sole carbon source. From this acclimated community, we isolated and identified by 16S rDNA analysis a strain of Acidovorax avenae, which was able to perform the complete biodegradation of 1,2-DCB in two days affording stoichiometric amounts of chloride. This pure strain was also tested for biodegradation of chlorobenzene (CB); 1,3-DCB and 1,4-DCB, giving similar results to the experiments using 1,2-DCB.The aromatic-ring-hydroxylating dioxygenase (ARHDO) alpha-subunit gene core, encoding the catalytic site of the large subunit of chlorobenzene dioxygenase, was detected by PCR amplification and confirmed by DNA sequencing. These results suggest that the isolated strain of A. avenae could use a catabolic pathway, via ARHDO system, leading to the formation of chlorocatecols during the first steps of biodegradation, with further chloride release and subsequent paths that showed complete substrate consumption. (C) 2005 Elsevier Ltd. All rights reserved.