Glomerular basement membrane injuries in IgA nephropathy evaluated by double immunostaining for α5(IV) and α2(IV) chains of type IV collagen and low-vacuum scanning electron microscopy
Glomerular basement membrane injuries in IgA nephropathy evaluated by double immunostaining for α5(IV) and α2(IV) chains of type IV collagen and low-vacuum scanning electron microscopy
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DOI：
10.1007/s10157-014-1008-8
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发表时间：
2015-06-01
影响因子：
2.3
通讯作者：
Shimizu, Akira
中科院分区：
文献类型：
--
作者：
Masuda, Yukinari;Yamanaka, Nobuaki;Shimizu, Akira
肾小球含有发达的毛细血管，由于高静水压、高滤过、高血压和炎症，这些毛细血管有损伤的风险。对50例(31.8+/-A17.6岁)IgA肾病患者肾活检标本进行IV型胶原α2(IV)和α5(IV)链双重免疫组织化学染色，并用透射电子显微镜和低真空扫描电子显微镜观察肾小球滤过屏障的超微结构改变。透射电子显微镜下可见基底膜变薄、裂隙、断裂、增厚，呈板层网状结构和双轮廓。α5(IV)和α2(IV)免疫组织化学双重染色显示基底膜增厚，α5(IV)减少，α2(IV)增加，或出现α5(IV)和α2(IV)的马赛克图像，并可见α5(IV)的空洞、骨折、棘突和断裂。此外，LV-SEM显示出蚀刻图像和多个孔在扩大和波浪形的基底膜。这些发现可能与IgA肾病肾小球基底膜脆性改变有关。IgA肾病肾小球基底膜的改变常见于IgA肾病，可通过IV型胶原α2(IV)和α5(IV)免疫组织化学双重染色和LV-SEM进行评估。将这些分析应用于人类肾活检标本，可能会增强我们对人类肾小球疾病中肾小球基底膜改变的理解。
The glomerulus contains well-developed capillaries, which are at risk of injury due to high hydrostatic pressure, hyperfiltration, hypertension and inflammation. However, the pathological alterations of the injured glomerular basement membrane (GBM), the main component of the glomerular filtration barrier, are still uncertain in cases of glomerulonephritis.We examined the alterations of the GBM in 50 renal biopsy cases with IgA nephropathy (31.8 +/- A 17.6 years old) using double immunostaining for the alpha 2(IV) and alpha 5(IV) chains of type IV collagen, and examining the ultrastructural alterations by transmission electron microscopy (TEM) and low-vacuum scanning electron microscopy (LV-SEM).The GBM of IgA nephropathy cases showed various morphological and qualitative alterations. In the TEM findings, thinning, gaps, rupture, thickening with a lamellar and reticular structure and double contours were detected in the GBM. Double immunostaining for alpha 5(IV) and alpha 2(IV) showed thickening of the GBM with reduced alpha 5(IV) and increased alpha 2(IV), or mosaic images of alpha 5(IV) and alpha 2(IV), and holes, fractures, spiny projections and rupture of alpha 5(IV) in the GBM. In addition, LV-SEM showed an etched image and multiple holes in a widening and wavy GBM. These findings might be associated with the development of a brittle GBM in IgA nephropathy.Glomerular basement membrane alterations were frequently noted in IgA nephropathy, and were easily evaluated by double immunostaining for alpha 2(IV) and alpha 5(IV) of type IV collagen and LV-SEM. The application of these analyses to human renal biopsy specimens may enhance our understanding of the alterations of the GBM that occur in human glomerular diseases.