Determination of lysozyme at the nanogram level in chicken egg white using Resonance Rayleigh-scattering method with Cd-doped ZnSe quantum dots as probe
Determination of lysozyme at the nanogram level in chicken egg white using Resonance Rayleigh-scattering method with Cd-doped ZnSe quantum dots as probe
复制标题
DOI：
10.1016/j.snb.2011.04.058
复制
发表时间：
2011-10
影响因子：
8.4
通讯作者：
Z. Cai;Guoxiang Chen;Xi Huang;M. Ma
中科院分区：
文献类型：
--
作者：
Z. Cai;Guoxiang Chen;Xi Huang;M. Ma
提出了一种以Cd掺杂的ZnSe量子点为探针，基于共振瑞利散射的高灵敏度测定溶菌酶的新方法。在水相中制备了谷胱甘肽包裹的Cd掺杂的ZnSe量子点。此外，还对量子点-溶菌酶体系的RRS光谱、透射电子显微镜和吸收光谱进行了表征。此外，还考察了溶液的pH值、量子点的用量、各试剂的混合顺序以及共存物质等因素对散射强度的影响。此外，还初步探讨了Cd掺杂的ZnSe量子点-溶菌酶体系增强RRS的可能机制。测定溶菌酶具有良好的灵敏度，检出限为6.50×10~(-10)−~10g·−~(-1)。测定溶菌酶含量的回收率为97.1~101.6%，相对标准偏差为2.5~3.1%。结果表明，本研究建立的方法具有灵敏、快速、简便、耐受性好的特点，适用于合成蛋清和蛋清中溶菌酶的测定。
A new method for the determination of lysozyme with high sensitivity based on Resonance Rayleigh scattering (RRS) by using Cd doped ZnSe quantum dots (QDs) as a probe was proposed in this experiment. Cd doped ZnSe QDs capped with glutathione were prepared in the water phase. Further, the RRS spectrum, transmission electron microscope, and absorption spectrum of the QDs–lysozyme system have been characterized. In addition, the effects of several factors on scattering intensities were investigated, including pH value of solution, amount of QDs, mixing sequence of each reagent and the coexisting substances. Moreover, the possible mechanism for the RRS enhancement of Cd doped ZnSe QDs–lysozyme system was preliminary discussed. The RRS method for the determination of lysozyme has good sensitivity with the detection limits 6.5 × 10−10g mL−1. The contents of lysozyme were determined with recoveries of 97.1–101.6% and relativity standard deviation of 2.5–3.1%, respectively. It proved that the method established in our study is very sensitive, rapid, convenient and tolerant for the determination of lysozyme in synthetically and chicken egg white.