3D printed mold leachates in PDMS microfluidic devices
3D printed mold leachates in PDMS microfluidic devices
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DOI：
10.1038/s41598-020-57816-y
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发表时间：
2020-01-22
影响因子：
4.6
通讯作者：
Songsasen, Nucharin
中科院分区：
文献类型：
--
作者：
Ferraz, Marcia de Almeida Monteiro Melo;Nagashima, Jennifer Beth;Songsasen, Nucharin
上世纪90年代，随着聚二甲基硅氧烷(PDMS)和软光刻技术的引入，微流体领域发生了革命性的变化，几乎不再需要洁净室环境来制造器件。最近，3D打印已被引入制造用于软光刻的模具，这是唯一经常需要洁净室环境的步骤，以进一步支持PDMS微流控器件的快速成型。然而，大多数商用3D打印树脂的毒性已经确定，对于3D打印模具泄漏到PDMS中的组件的可能性知之甚少，这反过来会阻碍在设备中培养的细胞和/或组织。在本研究中，我们研究了通过立体光刻生产的3D打印模具是否可以将组件浸入PDMS，并将3D打印模具与更传统的SU-8模具进行了比较。在由广泛使用的PDMS预聚体：固化剂比例(10：1、15：1和20：1)制备的PDMS设备中孵育的水溶液中检测到不同的渗滤液，这些渗滤液被鉴定为来自树脂和催化剂物质。接下来，我们探索了在这些由3D打印模具生产的PDMS设备中培养细胞和组织的可能性，并经过适当的设备清洗和调节。重要的是，我们证明了所产生的PDMS设备支持HeLa细胞和卵巢组织的体外生理培养，结果优于静态传统培养。
The introduction of poly(dimethylsiloxane) (PDMS) and soft lithography in the 90's has revolutionized the field of microfluidics by almost eliminating the need for a clean-room environment for device fabrication. More recently, 3D printing has been introduced to fabricate molds for soft lithography, the only step for which a clean-room environment is still often necessary, to further support the rapid prototyping of PDMS microfluidic devices. However, toxicity of most of the commercial 3D printing resins has been established, and little is known regarding the potential for 3D printed molds to leak components into the PDMS that would, in turn, hamper cells and/or tissues cultured in the devices. In the present study, we investigated if 3D printed molds produced by stereolithography can leach components into PDMS, and compared 3D printed molds to their more conventional SU-8 counterparts. Different leachates were detected in aqueous solutions incubated in the resulting PDMS devices prepared from widely used PDMS pre-polymer:curing agent ratios (10:1, 15:1 and 20:1), and these leachates were identified as originating from resins and catalyst substances. Next, we explored the possibility to culture cells and tissues in these PDMS devices produced from 3D printed molds and after proper device washing and conditioning. Importantly, we demonstrated that the resulting PDMS devices supported physiological cultures of HeLa cells and ovarian tissues in vitro, with superior outcomes than static conventional cultures.