A Neutron Reflectometry Study of Human Serum Albumin Adsorption in Situ
A Neutron Reflectometry Study of Human Serum Albumin Adsorption in Situ
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人血清白蛋白原位吸附的中子反射法研究
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
S. Satija
中科院分区：
文献类型：
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作者：
§. A. Liebmann;L. M. Lander;A. D. Foster;W. Brittain;E. Vogler;C. F. M. and;S. Satija
反射计用于表征硅片支撑的 NH3+ 封端的自组装硅烷单层 (SAM) 和氧化硅 (SiOx) 表面与空气、水和人血清白蛋白 (HSA) 溶液的关系。空气中 NH3+ 封端的 SAM 的 X 射线反射计 (XR) 显示出 14 A 厚的密堆积硅烷单层，与相对于表面法线倾斜约 30° 的全反式延伸烃链堆积一致。如果假设不稳定质子与氘交换导致界面主要由 ND3+ 组成，则 NH3+ 表面相对于 D2O 的中子反射 (NR) 与 (XR) 结果一致。用 SiOx 表面相对于 D2O 获得的 NR 结果根据约 20 A 厚的多孔水合氧化物层进行解释。 HSA 的 NR 从 D2O 缓冲液吸附到 NH3+ 封端的 SAM 表面上，揭示了两层吸附机制。直接邻近固体表面的第一层厚度约为 40 A，基本上与本体液体浓度无关……
Reflectometry is used to characterize silicon-wafer-supported NH3+-terminated self-assembled silane monolayers (SAMs) and silicon oxide (SiOx) surfaces against air, water, and human serum albumin (HSA) solutions. X-ray reflectometry (XR) of the NH3+-terminated SAM in air revealed a 14 A thick, close-packed silane monolayer consistent with an all-trans extended hydrocarbon chain packing tilted about 30° with respect to the surface normal. Neutron reflectometry (NR) of the NH3+ surface against D2O was consistent with (XR) results if it was assumed that labile protons exchange with deuterium resulting in an interface comprised largely of ND3+. NR results obtained with the SiOx surface against D2O were interpreted in terms of a porous, hydrated oxide layer about 20 A thick. NR of HSA adsorbed from D2O buffer onto NH3+ terminated SAM surfaces revealed a two-layer adsorption regime. The first layer directly adjacent to the solid surface was about 40 A thick and was essentially independent of bulk liquid concent...