A nuclear envelop-associated baculovirus protein promotes intranuclear lipid accumulation during infection
A nuclear envelop-associated baculovirus protein promotes intranuclear lipid accumulation during infection
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DOI：
10.1016/j.virol.2019.04.006
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发表时间：
2019-06-01
期刊：
影响因子：
3.7
通讯作者：
Sako, Yasushi
中科院分区：
文献类型：
--
作者：
Nagamine, Toshihiro;Inaba, Takehiko;Sako, Yasushi
尽管杆状病毒在细胞核内产生病毒包膜已被广泛接受，但感染细胞中膜脂的重新分布尚未得到证实。在这里，我们描述了一种杆状病毒蛋白（Bm5/Ac13：更名为BION；杆状病毒蛋白与内核膜和外核膜相关），它定位于内核膜和外核膜，并表明核膜（NE）蛋白促进病毒诱导的核内结构，即外膜区（PR）的形成。与其在病毒包膜中的作用一致，PR被发现含有病毒膜蛋白和脂质，表明PR的形成是通过核内脂质积累进行的。由于缺乏PR，大约50%的感染了缺乏BION病毒的细胞没有表现出多面体的产生。BION与NE的关联而不是与PR的关联可能通过NE到PR的脂质转运促进PR和多面体的形成。
Although it has been well-accepted that baculoviruses produce a virus envelop within the nucleus, the redistribution of membrane lipids in infected cells has not been demonstrated. Here, we characterize a baculovirus protein (Bm5/Ac13: renamed BION; baculovirus protein associated with both the inner- and outer nuclear membranes) that localizes to both the inner- and outer nuclear membranes and show that the nuclear membrane (NE) protein promotes formation of a virus-induced intranuclear structure, the peristromal region (PR). Consistent with its role in virus envelopment, the PR was found to contain viral membrane proteins and lipids, suggesting PR formation proceeds through intranuclear lipid accumulation. About 50% of the cells infected with a bion-deficient virus exhibited no polyhedra production due to lack of the PR. Association of BION with the NE rather than the PR may contribute to the formation of the PR and polyhedra via NE-to-PR lipid transport.