Neuronal and glial cell changes are determined by retinal vascularization in retinopathy of prematurity
Neuronal and glial cell changes are determined by retinal vascularization in retinopathy of prematurity
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DOI：
10.1002/cne.21449
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发表时间：
2007-10-01
影响因子：
2.5
通讯作者：
Fletcher, Erica L.
中科院分区：
文献类型：
--
作者：
Downie, Laura E.;Pianta, Michael J.;Fletcher, Erica L.
我们描述了氧诱导视网膜病变的血管、神经元和胶质细胞的变化，这是早产儿视网膜病变(ROP)的一个模型。新生SD大鼠暴露于80%+/-2%氧气中至出生后第11天(ROP)，然后一直暴露于室内空气中(ROP)或整个过程中暴露于室内空气(对照组)。在光学显微镜下观察视网膜结构，然后在中央、中周和周围区域进行包埋后的免疫细胞化学检查。用胶质纤维酸性蛋白对Muller细胞进行免疫细胞化学鉴定。血管形成的程度是通过组织学来确定的。ROP导致内细胞层和丛状层显著变薄，在ROP动物的外周内核层变得更加明显(11.3%比25.4%)。无长突细胞的氨基酸水平在外周视网膜中特别脆弱；双极细胞表现出类似的变化，但不那么显着。穆勒细胞的谷氨酰胺水平升高，并且在外围胶质细胞最多。在对照组中，血管系统在P18时延伸到视网膜周围区域，而在ROP组中，血管系统没有延伸到周边区域。最显著的变化模式是ROP动物的中周“过渡区”。靠近血管的区域显示出与中央视网膜相似的神经化学性质，表明视网膜内部血液供应具有局部保护作用。我们发现，ROP会产生复杂的血管、神经和神经胶质变化，这些变化与视网膜内血管的接近程度有关。
We have characterized the vascular, neuronal, and glial changes in oxygen-induced retinopathy, a model of retinopathy of prematurity (ROP). Newborn Sprague-Dawley rats were exposed to either 80% +/- 2% oxygen to postnatal day P11 and then room air until P18 (ROP) or room air for the entire duration (controls). Retinal structure was examined under the light microscope and following postembedding immunocytochemistry in central, midperipheral, and peripheral regions. Muller cells were evaluated immunocytochemically with glial fibrillary acidic protein. The extent of vascularization was established histologically. ROP caused significant thinning of the inner cellular and plexiform layers, which became more pronounced in the peripheral inner nuclear layer of ROP animals (11.3% loss vs. 25.4% loss). Amacrine cell amino acid levels were particularly vulnerable in the peripheral retina; bipolar cells showed similar but less prominent changes. Muller cells had elevated glutamine levels and were most gliotic in the periphery. The vasculature extended to peripheral retinal regions at P18 in controls but not in ROP rats. The most striking pattern of change was evident in the midperipheral "transition zone" of ROP animals. Areas close to blood vessels showed neurochemical properties that were similar to those of the central retina, indicating a local protective effect of the inner retinal blood supply. We find that ROP produces complex vascular, neural, and glial changes that relate to the proximity of inner retinal blood vessels.