LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.
LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan.
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DOI：
10.1083/jcb.144.4.789
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发表时间：
1999-02-22
影响因子：
7.8
通讯作者：
Jackson, D G
中科院分区：
文献类型：
--
作者：
Banerji, S;Ni, J;Wang, S X;Clasper, S;Su, J;Tammi, R;Jones, M;Jackson, D G
细胞外基质糖胺聚糖透明质酸(HA)是皮肤和间充质组织中丰富的成分，在伤口愈合、炎症和胚胎形态形成过程中促进细胞迁移。在正常组织动态平衡期间，特别是在组织损伤后，HA通过淋巴管从这些部位动员到淋巴结，在那里被降解，然后进入循环，被肝脏快速摄取。然而，目前控制这一途径的HA结合分子的身份尚不清楚。在这里，我们描述了第一个这样的分子，LYVE-1，我们已经确定它是淋巴管壁上HA的主要受体。LYVE-1推导的氨基酸序列预测了一个322个残基的I型完整膜多肽，与CD44 HA受体有41%的相似性，其胞外结构域有212个残基，包含一个单一的Link模块，即Link蛋白超家族的原型HA结合域。与CD44一样，Lyve-1分子可结合可溶性和固定化HA。然而，与CD44不同的是，Lyve-1分子与HA共存于淋巴管壁的管腔表面，完全不存在于血管中。因此，LYVE-1是第一个被表征的淋巴特异性HA受体，是淋巴血管本身的唯一强大的标记物。
The extracellular matrix glycosaminoglycan hyaluronan (HA) is an abundant component of skin and mesenchymal tissues where it facilitates cell migration during wound healing, inflammation, and em- bryonic morphogenesis. Both during normal tissue homeostasis and particularly after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. Currently, however, the identities of HA binding molecules which control this pathway are unknown. Here we describe the first such molecule, LYVE-1, which we have identified as a major receptor for HA on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.