Oligosaccharide-assisted direct immunosensing of small molecules.
Oligosaccharide-assisted direct immunosensing of small molecules.
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DOI：
10.1021/ac100865p
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发表时间：
2010-06
影响因子：
7.4
通讯作者：
N. Kobayashi;H. Oyama;Iwao Suzuki;Y. Kato;Takeo Umemura;J. Goto
中科院分区：
文献类型：
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作者：
N. Kobayashi;H. Oyama;Iwao Suzuki;Y. Kato;Takeo Umemura;J. Goto
“三明治型”非竞争性（免疫测定）测定允许高灵敏度高通量大分子传感和测定，但不能用于小分子（半抗原）。在这里，我们从噬菌体展示库中分离出单链Fv片段，该片段与特定半抗原（维生素D和A衍生物）与固定化β-环糊精或β-麦芽糖基残基的复合物结合，并形成三元复合物。这些scFv使新的“半夹心型”免疫测定半抗原与纳摩尔范围的灵敏度。
"Sandwich-type" noncompetitive (immunometric) assays allow for high-sensitivity high-throughput macromolecule sensing and determination but cannot be used on small molecules (haptens). Here, we isolated single-chain Fv fragments from a phage-display library, which bound to complexes of particular haptens (vitamin D and A derivatives) with immobilized beta-cyclodextrin or beta-maltosyl residues, and formed ternary complexes. These scFvs enabled novel "semisandwich-type" immunometric assays of haptens with nanomole-range sensitivities.