Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes
Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes
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DOI：
10.1038/nbt1108
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发表时间：
2005-07-01
影响因子：
46.9
通讯作者：
Bowler, C
中科院分区：
文献类型：
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作者：
Davuluri, GR;van Tuinen, A;Bowler, C
番茄是类胡萝卜素和类黄酮的主要膳食来源，两者都对人类健康非常有益（1，2）。编码生物合成酶或转录因子的基因的过表达导致番茄具有改善的类胡萝卜素或类黄酮含量，但从未同时具有两者（3-7）。我们试图通过抑制内源性光形态建成调控基因DET 1，利用果实特异性启动子结合RNA干扰（RNAi）技术来提高番茄果实的营养价值。分子分析表明，DET 1的成绩单确实是专门降解转基因水果。类胡萝卜素和类黄酮含量显着增加，而果实品质的其他参数基本不变。这些结果表明，植物调控基因的操作可以同时影响从独立的生物合成途径产生的几种植物营养素的生产，并提供了一个新的例子，使用器官特异性基因沉默，以提高植物衍生产品的营养价值。
Tomatoes are a principal dietary source of carotenoids and flavonoids, both of which are highly beneficial for human health(1,2). Overexpression of genes encoding biosynthetic enzymes or transcription factors have resulted in tomatoes with improved carotenoid or flavonoid content, but never with both(3-7). We attempted to increase tomato fruit nutritional value by suppressing an endogenous photomorphogenesis regulatory gene, DET1, using fruit-specific promoters combined with RNA interference (RNAi) technology. Molecular analysis indicated that DET1 transcripts were indeed specifically degraded in transgenic fruits. Both carotenoid and flavonoid contents were increased significantly, whereas other parameters of fruit quality were largely unchanged. These results demonstrate that manipulation of a plant regulatory gene can simultaneously influence the production of several phytonutrients generated from independent biosynthetic pathways, and provide a novel example of the use of organ-specific gene silencing to improve the nutritional value of plant-derived products.