Genetic variation and phylogeography of central Asian and other house mice, including a major new mitochondrial lineage in Yemen.
Genetic variation and phylogeography of central Asian and other house mice, including a major new mitochondrial lineage in Yemen.
复制标题
中亚和其他家鼠的遗传变异和系统发育地理学，包括也门的一个主要的新线粒体谱系。
DOI：
10.1093/genetics/150.2.835
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Sage,RD
中科院分区：
文献类型：
--
作者：
Prager,EM;Orrego,C;Sage,RD
对从埃及、土耳其、也门、伊朗、阿富汗、巴基斯坦和尼泊尔到东亚的60个地点收集的76只小鼠的线粒体DNA（mtDNA）控制区和侧翼tRNA进行测序。Y染色体片段和加工过的p53假基因（p53）从许多这些小鼠和其他收集在欧亚大陆和北非的其他地方扩增。本文报道了251种线粒体DNA类型，包括54种新的类型，通过对这一片段进行测序，现已从小家鼠（Mus musculus group）中鉴定出251种线粒体DNA类型，可分为四个主要谱系：小家鼠、小家鼠、栗鼠和在也门发现的一个新谱系。进化树分析表明，中国人mtDNA是其他三个中国人mtDNA谱系的姐妹群，也门人mtDNA是下一个最古老的谱系。利用这棵树和地理学的方法，我们得出了一个新的模型的起源和辐射的rabsal家鼠的主要特点是起源于中西亚洲（在今天的范围内M。首先是阿拉伯半岛南部，然后向东和向北进入亚洲中南部，后来从亚洲中南部到亚洲中北部（并从那里进入欧亚北方的大部分地区）和东南亚。在伊朗和阿富汗的小鼠中检测到Zfy-2基因中有和没有18 bp缺失的Y染色体，而在土耳其，也门，巴基斯坦和尼泊尔只发现了未缺失的Y染色体。在格鲁吉亚、伊朗、土库曼斯坦、阿富汗和巴基斯坦观察到了p53的多态性。对79只成年小鼠的128 bp p53基因片段进行测序，发现12个变异位点，涉及≥14个等位基因。这种等位基因似乎是遗传学上的祖先，分布广泛，在土耳其、阿富汗、巴基斯坦和尼泊尔观察到最大的多样性。两个小鼠提供的证据，第二个p53基因座在一些哺乳动物种群。
The mitochondrial DNA (mtDNA) control region and flanking tRNAs were sequenced from 76 mice collected at 60 localities extending from Egypt through Turkey, Yemen, Iran, Afghanistan, Pakistan, and Nepal to eastern Asia. Segments of the Y chromosome and of a processed p53 pseudogene (ψp53) were amplified from many of these mice and from others collected elsewhere in Eurasia and North Africa. The 251 mtDNA types, including 54 new ones reported here, now identified from commensal house mice (Mus musculus group) by sequencing this segment can be organized into four major lineages—domesticus, musculus, castaneus, and a new lineage found in Yemen. Evolutionary tree analysis suggested the domesticus mtDNAs as the sister group to the other three commensal mtDNA lineages and the Yemeni mtDNAs as the next oldest lineage. Using this tree and the phylogeographic approach, we derived a new model for the origin and radiation of commensal house mice whose main features are an origin in west-central Asia (within the present-day range of M. domesticus) and the sequential spreading of mice first to the southern Arabian Peninsula, thence eastward and northward into south-central Asia, and later from south-central Asia to north-central Asia (and thence into most of northern Eurasia) and to southeastern Asia. Y chromosomes with and without an 18-bp deletion in the Zfy-2 gene were detected among mice from Iran and Afghanistan, while only undeleted Ys were found in Turkey, Yemen, Pakistan, and Nepal. Polymorphism for the presence of a ψp53 was observed in Georgia, Iran, Turkmenistan, Afghanistan, and Pakistan. Sequencing of a 128-bp ψp53 segment from 79 commensal mice revealed 12 variable sites and implicated ≥14 alleles. The allele that appeared to be phylogenetically ancestral was widespread, and the greatest diversity was observed in Turkey, Afghanistan, Pakistan, and Nepal. Two mice provided evidence for a second ψp53 locus in some commensal populations.