An aptamer-patterned hydrogel for the controlled capture and release of proteins via biorthogonal click chemistry and DNA hybridization.
An aptamer-patterned hydrogel for the controlled capture and release of proteins via biorthogonal click chemistry and DNA hybridization.
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DOI：
10.1039/c7tb00883j
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发表时间：
2017-08
期刊：
影响因子：
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通讯作者：
Zheng Zhang;Jie Du;Yu-li Li;Jincai Wu;Feng Yu;Yong Chen
中科院分区：
文献类型：
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作者：
Zheng Zhang;Jie Du;Yu-li Li;Jincai Wu;Feng Yu;Yong Chen
三维（3D）生物活性蛋白质图案化水凝胶提供了一个比传统的生物惰性支架细胞培养更仿生的环境。然而，已经证明难以获得具有同时图案化的蛋白质和在稍后阶段的受控释放而不使脆性蛋白质的生物活性失活的水凝胶。在本研究中，使用双正交“点击化学”形成适体图案化的PEG水凝胶。图案化适体可以基于适体与蛋白质之间的特异性亲和力选择性地捕获生物活性蛋白质，这导致在水凝胶内形成图案化蛋白质。更重要的是，通过加入适体的互补DNA链（cDNA），由于DNA杂交，蛋白质可以在任何期望的时间和浓度从适体解离。这种适体图案化的水凝胶可以成为一种智能和仿生的3D微环境，用于以时空方式同时控制蛋白质的递送。
Three-dimensional (3D) bioactive protein-patterned hydrogels provide a more biomimetic environment for cell culture than traditional bioinert scaffolds. However, it has proved difficult to obtain a hydrogel with simultaneously patterned proteins and controlled release at a later stage without inactivating the bioactivity of the fragile proteins. In this study, an aptamer-patterned PEG hydrogel was formed using biorthogonal "click chemistry". The patterned aptamer could selectively capture the bioactive protein based on the specific affinity between the aptamer and protein, which results in the formation of a patterned protein within the hydrogel. More importantly, by adding the complementary DNA strand (cDNA) of the aptamer, the protein can dissociate from the aptamer at any desired time and concentration due to the DNA hybridization. This aptamer-patterned hydrogel could become a smart and biomimetic 3D micro-environment for controlled delivery of proteins simultaneously in a spatiotemporal manner.