Cytomegalovirus reinfection in young children
Cytomegalovirus reinfection in young children
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DOI：
10.1016/s0022-3476(96)70279-2
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发表时间：
1996-03-01
影响因子：
5.1
通讯作者：
Murph, JR
中科院分区：
文献类型：
--
作者：
Bale, JF;Petheram, SJ;Murph, JR
目的：方法：对92例从儿童保健中心连续分离的巨细胞病毒（CMV）进行分析。从1986年至1994年，从锡达拉皮兹和爱荷华州爱荷华市的六个中心之一的37名儿童获得CMV菌株。通过基于聚合酶链反应的算法，使用针对人CMV的α-序列、糖蛋白B和主要立即早期（MIE）基因的引物，分析CMV分离株。的a-序列聚合酶链反应产物进行了比较的基础上的大小，和产品来自糖蛋白B和MIE基因进行了比较，根据限制性片段长度polymorphisms.Results：儿童之间的8个月和5岁7个月的年龄在CMV分离。每名儿童分离到2 ~ 6株CMV，首次分离与末次分离之间的间隔时间为11周至3年以上。37名儿童中至少有7名（19%）有一种以上CMV株感染的证据。在这些儿童中，6个不同的菌株的再感染证实了连续CMV strain.Conclusions的MIE基因产物的分析：儿童谁参加儿童保健中心，像成年人谁是免疫抑制或有多个性伴侣，是在被不同的CMV株再感染的风险。
Objective: To determine the frequency of reinfection with new cytomegalovirusMethods: Ninety-two CMV strains isolated serially from children attending child care centers were analyzed. Strains were obtained from 1986 to 1994, from 37 children attending one of six centers in the area of Cedar Rapids and lowa City, lowa, The CMV isolates were analyzed by a polymerase chain reaction-based algorithm using primers for the a-sequence, glycoprotein B, and major immediate early (MIE) genes of human CMV. The a-sequence polymerase chain reaction products were compared on the basis of size, and products derived from glycoprotein B and MIE genes were compared according to restriction fragment length polymorphisms.Results: Children were between 8 months and 5 years 7 months of age at the time of CMV isolation. The number of isolates ranged from 2 to 6 per child, and the intervals between the first and last CMV isolation ranged from 11 weeks to more than 3 years, At least 7 (19%) of the 37 children had evidence of infection with more than one CMV strain. In six of these children, reinfection with distinct strains was confirmed by analysis of the MIE gene products of sequential CMV strains.Conclusions: Children who attend child care centers, like adults who are immunosuppressed or have multiple sexual partners, are at risk of being-reinfected with distinct CMV strains.