Hybrid male sterility in mimulus (phrymaceae) is associated with a geographically restricted mitochondrial rearrangement
Hybrid male sterility in mimulus (phrymaceae) is associated with a geographically restricted mitochondrial rearrangement
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DOI：
10.1111/j.1558-5646.2008.00360.x
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发表时间：
2008-05-01
期刊：
影响因子：
3.3
通讯作者：
Willis, John H.
中科院分区：
文献类型：
--
作者：
Case, Andrea L.;Willis, John H.
细胞质雄性不育（CMS）和核育性恢复（Rf）涉及基因组间的协同进化。虽然雄性不育表型很少在被子植物的自然群体中表达，但CMS基因被认为是常见的。CMS/Rf系统的进化动力学知之甚少，留下的差距，我们的理解的机制和后果的细胞核相互作用。本文研究了一个小斑CMS基因的分子基础和地理分布。我们用异交M. guttatus（具有CMS和Rf）至自交不亲和M. nasutus（缺乏CMS和Rf）以产生CMS分离的杂种。含有必需基因（nad 6）的线粒体转录本与雄性不育完全相关。M. nasutus，存在于从原始收集点收集的所有基因型中，但仅在来自34个其他M. guttatus种群。这种模式表明，CMS可能起源于一个单一的地方，传播到固定的人口，但没有蔓延到其他人口，这表明可能的生态或遗传的限制，这CMS有丝分裂型人口之间的传播。极端本地化可能是雌雄同体物种CMS的特征，与在地理上广泛分布的有丝分裂型在雌雄异株物种中常见的相反，并且可能直接导致自然界中的杂交不相容性。
Cytoplasmic male sterility (CMS) and nuclear fertility restoration (Rf) involves intergenomic coevolution. Although male-sterile phenotypes are rarely expressed in natural populations of angiosperms, CMS genes are thought to be common. The evolutionary dynamics of CMS/Rf systems are poorly understood, leaving gaps in our understanding of mechanisms and consequences of cytonuclear interactions. We characterized the molecular basis and geographic distribution of a CMS gene in Mimulus guttatus. We used outcrossing M. guttatus (with CMS and Rf) to self-fertilizing M. nasutus (lacking CMS and Rf) to generate hybrids segregating for CMS. Mitochondrial transcripts containing an essential gene (nad6) were perfectly associated with male sterility. The CMS mitotype was completely absent in M. nasutus, present in all genotypes collected from the original collection site, but in only two individuals from 34 other M. guttatus populations. This pattern suggests that the CMS likely originated at a single locality, spread to fixation within the population, but has not spread to other populations, indicating possible ecological or genetic constraints on dispersal of this CMS mitotype between populations. Extreme localization may be characteristic of CMS in hermaphroditic species, in contrast to geographically widespread mitotypes commonly found in gynodioecious species, and could directly contribute to hybrid incompatibilities in nature.