Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3.
Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3.
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二维VOPBA揭示了层粘连蛋白受体（LAMR1）与登革热病毒血清型1、2和3的相互作用。
DOI：
10.1186/1743-422x-2-25
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发表时间：
2005-03-25
期刊：
影响因子：
4.8
通讯作者：
Cardosa, Mary Jane
中科院分区：
文献类型：
--
作者：
Tio, Phaik Hooi;Jong, Wan Wui;Cardosa, Mary Jane
登革病毒受体的研究产生了许多候选者，通常仅通过分子量来鉴定。病毒在体外的广泛宿主范围与鉴定受体的多种方法相结合，导致了可能涉及许多受体或附着蛋白的概念，并且不同的登革病毒血清型可能利用相同细胞以及不同细胞类型上的不同受体。在这项研究中，我们使用去污剂β-辛基吡喃葡萄糖苷，然后脱氧胆酸钠连续提取PS克隆D细胞单层，以制备富含细胞膜的组分。然后，我们使用2维（2D）凝胶电泳分离膜蛋白，并应用一种改良的病毒覆盖蛋白结合试验（VOPBA），以显示登革热病毒血清型1，2和3都与37 kDa/67 kDa层粘连蛋白受体（LAMR 1），一种常见的非整合素表面蛋白在许多细胞类型的相互作用。4种登革热血清型中至少有3种与37 kDa/67 kDa层粘连蛋白受体LAMR 1相互作用，LAMR 1可能是登革热病毒-细胞表面相互作用的共同参与者。
The search for the dengue virus receptor has generated many candidates often identified only by molecular mass. The wide host range of the viruses in vitro combined with multiple approaches to identifying the receptor(s) has led to the notion that many receptors or attachment proteins may be involved and that the different dengue virus serotypes may utilize different receptors on the same cells as well as on different cell types. In this study we used sequential extraction of PS Clone D cell monolayers with the detergent β-octylglucopyranoside followed by sodium deoxycholate to prepare a cell membrane-rich fraction. We then used 2 dimensional (2D) gel electrophoresis to separate the membrane proteins and applied a modified virus overlay protein binding assay (VOPBA) to show that dengue virus serotypes 1, 2 and 3 all interact with the 37 kDa/67 kDa laminin receptor (LAMR1), a common non-integrin surface protein on many cell types. At least 3 of the 4 dengue serotypes interact with the 37 kDa/67 kDa laminin receptor, LAMR1, which may be a common player in dengue virus-cell surface interaction.