The ABC transporter gene family of Daphnia pulex.
The ABC transporter gene family of Daphnia pulex.
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DOI：
10.1186/1471-2164-10-170
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发表时间：
2009-04-21
期刊：
影响因子：
4.4
通讯作者：
Dean M
中科院分区：
文献类型：
--
作者：
Sturm A;Cunningham P;Dean M
ABC（ATP结合盒）转运蛋白的大基因超家族编码参与跨生物膜的运输过程和进一步的基本细胞生物学功能的膜蛋白。ABC转运蛋白在进化上是古老的，参与了对有毒物质的生化防御。在这里，我们报告了一个全基因组的调查蚤状水蚤的ABC蛋白，第一次提供信息的ABC蛋白在甲壳类，主要是水生节肢动物亚门的高生态和经济的重要性。我们在水蚤基因组中鉴定了64种ABC蛋白，它们拥有所有目前ABC亚科A到H的成员。为了阐明系统发育关系，将水蚤的ABC蛋白与酵母、蠕虫、果蝇和人类的ABC蛋白进行了比较。水蚤中ABC转运蛋白的高度保守性表现在参与细胞基本过程的蛋白质上，包括ABCB亚家族的线粒体半转运蛋白，其在铁代谢和Fe/S蛋白前体的转运中起作用，以及ABCD、ABCE和ABCF亚家族的成员，其分别在极长链脂肪酸转运、基因转录起始和蛋白质翻译中起作用。一些水蚤蛋白显示一对一的orthopathic关系果蝇ABC蛋白，包括磺酰脲受体（SUR），蜕皮激素转运蛋白ET 23，和眼睛色素前体转运蛋白猩红色。作为果蝇，水蚤缺乏TAP蛋白的同源物，TAP蛋白在抗基因加工中起作用，囊性纤维化跨膜传导调节因子（CFTR）起氯离子通道的作用。水蚤中有2个与MDR（multidrug resistance）P-糖蛋白同源的蛋白（ABCB亚家族）和6个与MRP（multidrug resistance-associated proteins）同源的蛋白（ABCC亚家族）。然而，在ABCB和ABCC亚家族中谱系特异性基因重复使功能推断复杂化。在ABCG和ABCH亚家族中观察到特别高数量的基因重复，它们分别有23个和15个成员。蚤状水蚤基因组中ABC转运蛋白的计算机表征显示，甲壳动物中ABC转运蛋白的补体与其他后生动物一样复杂。毫不奇怪，在目前可用的基因组中，水蚤ABC转运蛋白与另一种节肢动物果蝇的转运蛋白最相似。
The large gene superfamily of ABC (ATP-binding cassette) transporters encodes membrane proteins involved in trafficking processes across biological membranes and further essential cell biological functions. ABC transporters are evolutionary ancient and involved in the biochemical defence against toxicants. We report here a genome-wide survey of ABC proteins of Daphnia pulex, providing for the first time information on ABC proteins in crustacea, a primarily aquatic arthropod subphylum of high ecological and economical importance. We identified 64 ABC proteins in the Daphnia genome, which possesses members of all current ABC subfamilies A to H. To unravel phylogenetic relationships, ABC proteins of Daphnia were compared to those from yeast, worm, fruit fly and human. A high conservation of Daphnia of ABC transporters was observed for proteins involved in fundamental cellular processes, including the mitochondrial half transporters of the ABCB subfamily, which function in iron metabolism and transport of Fe/S protein precursors, and the members of subfamilies ABCD, ABCE and ABCF, which have roles in very long chain fatty acid transport, initiation of gene transcription and protein translation, respectively. A number of Daphnia proteins showed one-to-one orthologous relationships to Drosophila ABC proteins including the sulfonyl urea receptor (SUR), the ecdysone transporter ET23, and the eye pigment precursor transporter scarlet. As the fruit fly, Daphnia lacked homologues to the TAP protein, which plays a role in antigene processing, and the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as a chloride channel. Daphnia showed two proteins homologous to MDR (multidrug resistance) P-glycoproteins (ABCB subfamily) and six proteins homologous to MRPs (multidrug resistance-associated proteins) (ABCC subfamily). However, lineage specific gene duplications in the ABCB and ABCC subfamilies complicated the inference of function. A particularly high number of gene duplications were observed in the ABCG and ABCH subfamilies, which have 23 and 15 members, respectively. The in silico characterisation of ABC transporters in the Daphnia pulex genome revealed that the complement of ABC transporters is as complex in crustaceans as that other metazoans. Not surprisingly, among currently available genomes, Daphnia ABC transporters most closely resemble those of the fruit fly, another arthropod.
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DOI：
10.1073/pnas.0603098103
发表时间：
2006-08-08
影响因子：
11.1
作者：
Akasaka, Takeshi;Klinedinst, Susan;Bodmer, Rolf
通讯作者：
Bodmer, Rolf
影响因子：
4.8
作者：
Chen, ZQ;Dong, JS;Dean, M
通讯作者：
Dean, M
影响因子：
64.8
作者：
Dawson, Roger J. P.;Locher, Kaspar P.
通讯作者：
Locher, Kaspar P.
DOI：
10.1073/pnas.95.26.15665
发表时间：
1998-12-22
影响因子：
11.1
作者：
Doyle, LA;Yang, WD;Ross, DD
通讯作者：
Ross, DD
影响因子：
2.9
作者：
Aurade, Ravindra;Jayalakshmi, Senigala K.;Sreeramulu, Kuruba
通讯作者：
Sreeramulu, Kuruba