NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network
NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network
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DOI：
10.1111/j.1365-2443.2005.00894.x
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发表时间：
2005-10-01
期刊：
影响因子：
2.1
通讯作者：
Murata, M
中科院分区：
文献类型：
--
作者：
Kano, F;Kondo, H;Murata, M
内质网（ER）具有特征性的多边形结构，具有标志性的三方连接。在先前的论文中，我们使用来自HeLa细胞的有丝分裂胞质溶胶在链球菌溶血素O（SLO）透化的CHO-HSP细胞（稳定表达GFP-HSP 47）中重建了预先存在的ER网络的破坏。此外，我们发现间期胞质溶胶诱导重组的中断ER网络成一个连续的网络结构。在这里，我们表明，重组的ER网络是通过两个连续的融合反应。第一个过程是由NSF/α和γ-SNAP介导的，并且涉及连接被破坏的ER小管的典型膜中间结构的产生。随后的融合是由p97/p47/VCIP 135介导的，这已被证明是有丝分裂后高尔基体再生长中的同型融合事件所必需的。此外，我们还发现这两个融合过程都涉及t-SNARE，syntaxin 18。
The endoplasmic reticulum (ER) has a characteristic polygonal structure with hallmark three-way junctions. In a previous paper, we reconstituted the disruption of the pre-existing ER network using mitotic cytosol from HeLa cells in streptolysin O (SLO)-permeabilized CHO-HSP cells (stably expressing GFP-HSP47). In addition, we found that interphase cytosol induced reformation of the disrupted ER network into a continuous network structure. Here, we show that the reformation of the ER network is accomplished through two sequential fusion reactions. The first process is mediated by NSF/alpha and gamma-SNAPs, and involves the generation of typical membranous intermediate structures that connect the disrupted ER tubules. A subsequent fusion is mediated by p97/p47/VCIP135, which has been shown to be required for homotypic fusion events in Golgi cisternae regrowth after mitosis. In addition, we also found that both fusion processes involve the t-SNARE, syntaxin 18.