Effects of red ginseng on the elastic properties of human skin
Effects of red ginseng on the elastic properties of human skin
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DOI：
10.1016/j.jgr.2019.08.004
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发表时间：
2020-09-01
影响因子：
6.3
通讯作者：
Kim，Kyung Sook
中科院分区：
文献类型：
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作者：
Park，Moon Young;Han，Se Jik;Kim，Kyung Sook
背景红参含有微量元素、维生素、精油和脂肪酸等成分，可用于护肤品以延缓衰老过程。我们通过评估细胞硬度和测量胶原蛋白合成来研究红参治疗对皮肤弹性的影响。方法用红参处理人真皮成纤维细胞，并使用原子力显微镜研究由此产生的硬度变化。研究了衰老生物标志物的细胞骨架变化和 mRNA 表达，包括前胶原 I 和 VII、弹性蛋白和原纤维蛋白-1。通过苏木精和伊红染色、扫描电子显微镜和原子力显微镜观察用红参处理的人体皮肤等效物中的胶原蛋白。结果和结论用≥ 0.8 mg/mL 的红参浓度处理后，成纤维细胞的硬度显着降低。治疗后 F-肌动蛋白与 G-肌动蛋白的比率下降，这对应于成纤维细胞硬度的变化。红参处理降低了皮肤等效物的储能模量（G'）和损耗模量（G''）。该结果表明，通过红参治疗可以恢复皮肤等效物的粘弹性。
BackgroundRed ginseng contains components, including microelements, vitamins, essential oils, and fatty acids, that can be used in skincare to delay the aging process. We investigated the effects of red ginseng treatment on skin elasticity by assessing cellular stiffness and measuring collagen protein synthesis.MethodsHuman dermal fibroblasts were treated with red ginseng, and the resulting changes in stiffness were investigated using atomic force microscopy. Cytoskeletal changes and mRNA expression of biomarkers of aging, including that of procollagens I and VII, elastin, and fibrillin-1, were investigated. Collagen in a human skin equivalent treated with red ginseng was visualized via hematoxylin and eosin staining, scanning electron microscopy, and atomic force microscopy.Results and conclusionThe stiffness of fibroblasts was significantly reduced by treatment with red ginseng concentrations of ≥ 0.8 mg/mL. The ratio of F-actin to G-actin decreased after treatment, which corresponded to a change in fibroblast stiffness. The storage modulus (G′) and loss modulus (G″) of the skin equivalent were both lowered by red ginseng treatment. This result indicates that the viscoelasticity of the skin equivalent can be restored by red ginseng treatment.