Low doses ionizing radiation enhances the invasiveness of breast cancer cells by inducing epithelial-mesenchymal transition
Low doses ionizing radiation enhances the invasiveness of breast cancer cells by inducing epithelial-mesenchymal transition
复制标题
低剂量电离辐射通过诱导上皮间质转化增强乳腺癌细胞的侵袭力
DOI：
10.1016/j.bbrc.2011.07.074
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发表时间：
2011-08-19
影响因子：
3.1
通讯作者：
Moran, Meena S.
中科院分区：
文献类型：
--
作者：
Zhang, Xin;Li, Xiaoyan;Moran, Meena S.
上皮间质转化（EMT）是细胞形态和分子改变促进细胞侵袭的过程。我们假设低剂量电离辐射 (LDIR) 通过诱导 EMT 来增强乳腺癌细胞的侵袭性。通过蛋白质印迹/RT-PCR 分析 LDIR 对 MCF-7 乳腺癌细胞形态和 EMT 标志物的影响，并使用 Transwell 实验检查迁移/侵袭。我们发现LDIR导致MCF-7细胞EMT的表型变化以及上皮分化标记物的下调和间充质标记物的转录诱导。此外，与未辐射的细胞相比，辐射的细胞表现出增强的迁移/侵袭 MCF-7 细胞。总之，LDIR通过上皮向间质转化促进乳腺癌细胞的侵袭性。这些发现可能最终为提高乳腺癌放射治疗效果提供一种新的靶向方法。 (C) 2011 Elsevier Inc. 保留所有权利。
Epithelial-mesenchymal transition (EMT) is a process cellular morphologic and molecular alterations facilitate cell invasion. We hypothesized that low dose ionizing irradiation (LDIR) enhances the invasiveness of breast cancer cells by inducing EMT. The effects of LDIR on cellular morphology and the EMT markers of MCF-7 breast cancer cells were analyzed by western blot/RT-PCR and migration/invasion was examined using the transwell assay. We found that LDIR led to the phenotypic changes of EMT in MCF-7 cells and down-regulation of epithelial differentiation markers and transcriptional induction of mesenchymal markers. Furthermore, the radiated cells demonstrated enhanced migration/invasion MCF-7 cells compared with non-radiated cells. In summary, LDIR promotes the invasiveness of breast cancer cells through epithelial to mesenchymal transition. These findings may ultimately provide a new targeted approach for improving the therapeutic effectiveness of radiation in breast cancer. (C) 2011 Elsevier Inc. All rights reserved.