Basophils in the giant papillae of chronic allergic keratoconjunctivitis
Basophils in the giant papillae of chronic allergic keratoconjunctivitis
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DOI：
10.1136/bjo.2009.165316
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发表时间：
2010-04-01
影响因子：
4.1
通讯作者：
Murakami, A.
中科院分区：
文献类型：
--
作者：
Matsuda, A.;Ebihara, N.;Murakami, A.
目的在小鼠模型中阐明嗜碱性粒细胞作为慢性变态反应起始物的重要作用。本研究的目的是分析嗜碱性粒细胞和其他相关的炎症细胞在慢性过敏性角结膜炎（AKC）和春季角结膜炎（VKC）患者的巨乳头进行透射电子显微镜（TEM）分析，以检查嗜碱性粒细胞的存在。巨乳头冰冻切片用嗜碱性粒细胞特异性抗体BB-1、抗CD 4、抗CD 8、抗CD 20、抗主要碱性蛋白（MBP）、抗IgE和抗Fc γ RI-b抗体免疫组化染色。在巨乳头的固有质中观察到小簇嗜碱性粒细胞，尤其是在血管内皮附近和上皮下区域。BB-1阳性的嗜碱性粒细胞簇被T细胞、B细胞、IgE阳性细胞和MBP阳性的嗜酸性粒细胞包围。结论嗜碱性粒细胞可从巨乳头血管内皮细胞向巨乳头浸润。嗜碱性粒细胞在炎性细胞中心的存在表明嗜碱性粒细胞作为慢性过敏性结膜炎的引发剂的作用。
Aims The essential role of basophils as an initiator of chronic allergic reaction has been elucidated in mouse models. The aim of this present study was to analyse the in situ immunolocalisation of basophils and other relevant inflammatory cells in chronic allergic keratoconjunctivitis.Methods Transmission electron microscopic (TEM) analysis was carried out to examine the existence of basophils in the giant papillae obtained from atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) patients. Cryostat sections of giant papillae were immunostained with basophil-specific antibody BB-1, and with anti-CD4, anti-CD8, anti-CD20, anti-major basic protein (MBP), anti-IgE and anti-Fc epsilon RI-b antibodies.Results TEM analysis confirmed the existence of basophils in the giant papillae. Small clusters of basophils were observed in the substantia propria of giant papillae, especially at the vicinity of vascular endothelium and subepithelial regions. BB-1-positive basophil clusters were surrounded by T cells, B cells, IgE-positive cells and MBP-positive eosinophils. No BB-1-positive basophils were observed in the control conjunctivae.Conclusion Basophils may infiltrate from either vascular endothelium into the giant papillae. The existence of basophils at the centre of inflammatory cells suggests the role of basophils as an initiator of chronic allergic conjunctivitis.