DEVELOPMENT, DIFFERENTIATION, AND MATURATION OF MACROPHAGES IN THE FETAL MOUSE-LIVER
DEVELOPMENT, DIFFERENTIATION, AND MATURATION OF MACROPHAGES IN THE FETAL MOUSE-LIVER
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DOI：
10.1002/jlb.48.1.27
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发表时间：
1990-07-01
影响因子：
5.5
通讯作者：
NISHIKAWA, SI
中科院分区：
文献类型：
--
作者：
NAITO, M;TAKAHASHI, K;NISHIKAWA, SI
妊娠10天时，在肝脏造血开始之前，原始巨噬细胞出现在胎鼠肝脏的窦腔中，并成熟为胎儿巨噬细胞。用LP3条件培养基培养胎肝细胞悬液，形成单核细胞集落，但体内肝脏造血早期单核细胞生成能力较差。在妊娠10天时从胎儿肝脏获得的细胞悬液在小鼠骨髓基质细胞系ST2的单层培养物中，在单核细胞/巨噬细胞集落发育之前形成原始/胎儿巨噬细胞集落，并且显示原始巨噬细胞向胎儿巨噬细胞的分化，而没有经过幼单核细胞和单核细胞阶段。此时，胎儿心血管系统在肝脏形成之前与卵黄静脉相连。随着妊娠的进展，观察到单核细胞系列发育并形成单核细胞/巨噬细胞群。使用 LP3 条件培养基和单层 ST2 进行的体外研究证实了这一点。因此，肝脏造血过程中似乎存在两种不同的巨噬细胞群，即原始/胎儿巨噬细胞群和单核细胞/巨噬细胞群。胎儿巨噬细胞似乎也与原始巨噬细胞不同，原始巨噬细胞从卵黄囊定植到胎儿肝脏中，或者可能由造血干细胞局部发育。
Primitive macrophages emerged in the sinusoidal lumen of the fetal mouse liver at 10 days of gestation before the initiation of hepatic hematopoiesis and matured into fetal macrophages. In the culture of cell suspensions from the fetal liver with LP3-conditioned medium, monocyte colonies were formed, but monocytopoiesis was poor in the early stage of hepatic hematopoiesis in vivo. In the culture of cell suspensions obtained from the fetal liver at 10 days of gestation on the monolayer of a mouse bone marrow stromal cell line, ST2, primitive/fetal macrophage colonies were formed before the development of monocyte/macrophage colonies and showed differentiation of primitive macrophages into fetal macrophages without passing through the stage of promonocytes and monocytes. At this time, the fetal cardiovascular system was connected with the vitelline vein just before the formation of the liver. With the progress of gestation, a monocytic cell series was observed to develop and form a monocyte/macrophage population. This was confirmed by in vitro studies with an LP3-conditioned medium and on a monolayer of ST2. Thus, it appears that there exist two different macrophage populations, a primitive/fetal macrophage population and a monocyte/macrophage population in hepatic hematopoiesis. It also appears that fetal macrophages are differentiated from primitive macrophages which are colonized into the fetal liver from the yolk sac or which develop in loco, presumably from hematopoietic stem cells.