Characterization of histone inheritance patterns in the Drosophila female germline.
Characterization of histone inheritance patterns in the Drosophila female germline.
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DOI：
10.15252/embr.202051530
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发表时间：
2021-07-05
期刊：
影响因子：
7.7
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Kahney EW;Zion EH;Sohn L;Viets-Layng K;Johnston R;Chen X
干细胞具有进行不对称分裂的独特能力，其产生两个遗传上相同的子细胞，但致力于不同的细胞命运。失去这种平衡的不对称结果可能导致许多疾病，包括癌症和组织营养不良。了解这种严格调控的过程对于开发治疗这些异常的方法至关重要。在这里，我们报告说，在果蝇雌性生殖干细胞不对称分裂，两个子细胞差异继承组蛋白的关键基因相关的要么维持干细胞状态或促进分化，但不是在组成性活跃或沉默的基因。我们将联合收割机组蛋白标记与DNA寡聚体结合起来，以区分新旧组蛋白，并在体内不对称分裂细胞中以单基因分辨率可视化其遗传模式。这种策略可以应用于其他生物系统，涉及细胞命运的变化在发展过程中或组织稳态多细胞生物体。这项研究显示了果蝇雌性生殖系中局部非重叠的旧组蛋白H3与新组蛋白H3。具有不对称H3遗传的基因组区域具有来自不对称干细胞分裂的两个子细胞之间的差异表达基因。
Stem cells have the unique ability to undergo asymmetric division which produces two daughter cells that are genetically identical, but commit to different cell fates. The loss of this balanced asymmetric outcome can lead to many diseases, including cancer and tissue dystrophy. Understanding this tightly regulated process is crucial in developing methods to treat these abnormalities. Here, we report that during a Drosophila female germline stem cell asymmetric division, the two daughter cells differentially inherit histones at key genes related to either maintaining the stem cell state or promoting differentiation, but not at constitutively active or silenced genes. We combine histone labeling with DNA Oligopaints to distinguish old versus new histones and visualize their inheritance patterns at a single‐gene resolution in asymmetrically dividing cells in vivo. This strategy can be applied to other biological systems involving cell fate change during development or tissue homeostasis in multicellular organisms. This study shows locally non‐overlapping old versus new histone H3 in the Drosophila female germline. The genomic regions with asymmetric H3 inheritance harbor differentially expressed genes between the two daughter cells derived from asymmetric stem cell divisions.