GENETIC POPULATION-STRUCTURE, CLONAL PHYLOGENY, AND PATHOGENICITY OF SALMONELLA-PARATYPHI B
GENETIC POPULATION-STRUCTURE, CLONAL PHYLOGENY, AND PATHOGENICITY OF SALMONELLA-PARATYPHI B
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DOI：
10.1128/iai.58.6.1891-1901.1990
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发表时间：
1990-06-01
影响因子：
3.1
通讯作者：
WHITTAM, TS
中科院分区：
文献类型：
--
作者：
SELANDER, RK;BELTRAN, P;WHITTAM, TS
通过对123株乙型副伤寒B型（血清型1，4，[5]，1,2：B：[1，2]）沙门菌染色体24个酶基因位点的等位变异分析，探讨其遗传多样性和亲缘关系。14个电泳类型，标记克隆，区分，克隆谱系的遗传重建，生物型和其他表型性状映射到这个结构。大多数d-酒石酸阴性菌株是一个丰富的，全球分布的克隆（铅1），是多态性的许多生物型特征（包括d-酒石酸利用率），噬菌体类型，rRNA模式，大肠杆菌素M和噬菌体ES 18的敏感性的成员。这个克隆体主要负责S。人类的副伤寒B肠热病。相比之下，d-酒石酸阳性strians（以前称为沙门氏菌爪哇）发生在所有7个克隆谱系确定的人口遗传分析，虽然大多数d-酒石酸阳性分离株属于只有两个克隆（Pb 3和PB 4），这在地理上的频率不同。单相菌株代表四个密切相关的克隆，形成一个独特的系统发育谱系。Kauffmann假说认为，通过重组（通过噬菌体转导或其他方法），远亲细胞谱系之间的血清型趋同可能解释了S. B型副伤寒。Pb 4、PB 6和Pb 7与鼠伤寒沙门氏菌和圣保罗沙门氏菌的克隆的亲缘关系比与沙门氏菌的其它克隆的亲缘关系更近。B型副伤寒。对大肠杆菌素M和噬菌体ES 18的敏感性或抗性以及rRNA的电泳图谱，这些都被纳入最近提出的用于鉴定S.乙型副伤寒B，单独或组合不能标记克隆或其它有意义的系统发生亚群。
Genetic diversity and relationship among 123 strains of Salmonella paratyphi B (serotype 1,4,[5],12:b:[1,2]) were estimated from an assessment of electrophoretically demonstrable allelic variation at 24 chromosomal enzyme gene loci. Fourteen electrophoretic types, marking clones, were distinguished, the phylogeny of the clonal lineages was reconstructed, and biotype and other phenotypic characters were mapped onto this structure. Most d-tartrate-negative strains are members of an abundant, globally distributed clone (Pb 1) that is polymorphic for many biotype characters (including d-tartrate utilization), bacteriophage type, rRNA pattern, and colicin M and phage ES18 sensitivity. This clone is largely responsible for S. paratyphi B enteric fever in humans. In contrast, d-tartrate-positive strians (formerly known as Salmonella java) occurred in all seven of the clonal lineages identified by population genetic analysis, although most d-tartrate-positive isolates belong to only two clones (Pb 3 and PB 4), which vary in frequency geographically. Monophasic strains represent four closely related clones forming a distinctive phylogenetic lineage. The Kauffmann hypothesis of convergence in serotype among distantly related cell lineages through recombination (via phage transduction or other means) may account for the considerable genotypic diversity among clones of S. paratyphi B. Pb 4, PB 6, and Pb 7 are more closely allied with clones of Salmonella typhimurium and Salmonella saintpaul than with other clones of S. paratyphi B. Sensitivity or resistance to colicin M and phage ES18 and the electrophoretic pattern of the rRNA, which were incorporated into a recently proposed scheme for the identification of types of S. paratyphi B, individually or in combination fail to mark clones or other meaningful phylogenetic subdivisions.