METHYLATION AND PROCESSING OF RIBOSOMAL RNA IN HELA CELLS
METHYLATION AND PROCESSING OF RIBOSOMAL RNA IN HELA CELLS
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DOI：
10.1016/0022-2836(66)90025-8
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发表时间：
1966-01-01
影响因子：
5.6
通讯作者：
PENMAN, S
中科院分区：
文献类型：
--
作者：
GREENBERG, H;PENMAN, S
已经研究了HeLa细胞中RNA的甲基化。掺入放射性甲基标记的甲硫氨酸后，将细胞分成细胞质、细胞核和核仁部分。高分子量RNA的甲基化仅发生在45 s核糖体RNA前体中，并发生在核仁中。45 s RNA的甲基化显然发生在新合成RNA的生长点附近，这一点可以通过在非常短的脉冲标记后存在甲基化的部分完成的分子来证明。用非放射性甲硫氨酸追踪甲基标记的RNA，并通过细胞组分跟踪核糖体RNA前体的进展。这些数据支持了先前发表的假说，即核仁45 s RNA是细胞质18 s RNA和核仁32 s RNA的前体。32 s RNA转化为28 s RNA，然后在核质中发现，并且是细胞质28 s RNA的明显前体。
The methylation of RNA in HeLa cells has been studied. After incorporation of radioactive methyl-labeled methionine, the cells were fractionated into cytoplasmic, nuclear, and nucleolar fractions. Methylation of high molecular weight RNA is shown to be exclusively in the 45 s ribosomal RNA precursor and takes place in the nucleolus. The methylation of the 45 s RNA apparently occurs close to the growing point of newly synthesized RNA as evidenced by the existence of methylated, partially completed molecules after very short pulse labeling. The methyl-labeled RNA was chased with non-radioactive methionine and the progression of the ribosomal RNA precursors was followed through the cell fractions. The data support the previously published hypothesis that nucleolar 45 s RNA is the precursor to cytoplasmic 18 s RNA and 32 s nucleolar RNA. The 32 s RNA is converted to 28 s RNA, which is then found in the nucleoplasm and is the apparent precursor to cytoplasmic 28 s RNA.