Isolation and characterization of polymorphic EST-SSR and genomic SSR markers in spotted mandarin fish (Siniperca scherzeri Steindachne).
Isolation and characterization of polymorphic EST-SSR and genomic SSR markers in spotted mandarin fish (Siniperca scherzeri Steindachne).
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DOI：
10.4238/2015.december.29.41
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Dou Yaqi;Xu-Fang Liang;Min Yang;C. Tian;Shan He;Wenjie Guo
中科院分区：
文献类型：
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作者：
Dou Yaqi;Xu-Fang Liang;Min Yang;C. Tian;Shan He;Wenjie Guo
斑点鳜鱼（sininiperca scherzeri Steindachne）一旦在野外开始进食，就只以其他鱼类的活鱼苗为食。本研究采用生物素富集方法，结合雪蚕（♀）与雪蚕（♂）F1种间杂交的转录组，在雪蚕中开发了26个多态性转录组衍生的SSR标记和14个基因组SSR标记，并对其进行了鉴定。这40个多态性ssr扩增了168个等位基因（平均4.2个）。等位基因数、观察杂合度、期望杂合度和多态性信息含量分别为2 ~ 7（平均4.3）、0.1111 ~ 1.000（平均0.6718）、0.3118 ~ 0.8276（平均0.6901）和0.2735 ~ 0.7902（平均0.6298）。经多次Bonferroni校正后，其中10个微卫星位点明显偏离Hardy-Weinberg平衡（P < 0.00125），未观察到显著的连锁不平衡（P < 0.00006）。该微卫星标记可以作为一种有价值的遗传评价工具，从遗传的角度分析雪氏弧菌独特的食物偏好机制。
Spotted mandarin fish (Siniperca scherzeri Steindachne) feed solely on live fry of other fish species once the fry start feeding in the wild. In the present study, 26 polymorphic transcriptome-derived simple sequence repeat (SSR) markers and 14 genomic SSR markers were developed and characterized in S. scherzeri Steindachne by combining a biotin-enrichment protocol and transcriptome of F1 interspecies hybrids between S. chuatsi (♀) and S. scherzeri (♂). These 40 polymorphic SSRs amplified 168 alleles (mean 4.2). The number of alleles, observed heterozygosity, expected heterozygosity, and polymorphic information content per locus were in the range of 2 to 7 (mean 4.3), 0.1111 to 1.000 (mean 0.6718), 0.3118 to 0.8276 (mean 0.6901), and 0.2735 to 0.7902 (mean 0.6298), respectively. Ten of these microsatellite loci deviated significantly from Hardy-Weinberg equilibrium (P < 0.00125) after Bonferroni correction for multiple tests and no significant linkage disequilibrium (P < 0.00006) was observed. The microsatellite markers characterized from S. scherzeri could be a valuable tool in genetic evaluation for conservation and for assessment of the mechanism associated with unique food preference of S. scherzeri from a genetic point of view.