EGG ALBUMIN EMBEDDING - A PROCEDURE COMPATIBLE WITH NEUROLOGICAL STAINING TECHNIQUES
EGG ALBUMIN EMBEDDING - A PROCEDURE COMPATIBLE WITH NEUROLOGICAL STAINING TECHNIQUES
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DOI：
10.3109/10520296309067158
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发表时间：
1963-01-01
期刊：
影响因子：
--
通讯作者：
DORSEY, CH
中科院分区：
文献类型：
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作者：
SNODGRESS, AB;DORSEY, CH
一种用于中枢神经系统和周围神经的冷冻切片的水溶性包埋介质，其将耐受载玻片上的酒精脱水、二甲苯清洁和合成树脂封固，可以通过将20克粉状卵清蛋白溶解在30毫升蒸馏水中来制备。将福尔马林固定、彻底清洗的组织浸入白蛋白溶液中数小时，然后包埋在其中。包埋时，用内层植物羊皮纸构建一个双层盒。它由铝箔外层支撑，铝箔外层底部有6-8个直径约0.5 mm的穿孔。当盒子在仅润湿盒子底部的25%福尔马林层中放置24-48小时时，通过羊皮纸进行福尔马林透析，实现组织-白蛋白块的硬化。此类块的冷冻切片与用于变性轴突的Nauta银浸渍、用于星形胶质细胞的Cajal氯化金升华和用于少突胶质细胞和小胶质细胞的Penfield改良碳酸银兼容。这些以及尼氏和髓鞘制备物显示最小的收缩和变形。
A water-soluble embedding medium for frozen sections of central nervous system and peripheral nerves which will tolerate on-the-slide alcohol dehydration, xylene clearing and synthetic resin mounting can be prepared by dissolbing 20 gm of powdered egg albumin in 30 ml of distilled water. Formalin-fixed, thoroughly washed tissue is immersed in the albumin solution for several hours and then embedded in it. For embedding, a two-layered box is constructed with an inner layer of vegetable parchment paper. This is supported by an outer layer of aluminum foil with 6-8 perforations about 0.5 mm in diameter in its bottom. Hardening of the tissue-albumin block is achieved by formalin dialysis through the parchment paper when the box is stood for 24-48 hr in a layer of 25% formalin that wets only the bottom of the box. Frozen sections from such blocks are compatible with Nauta silver impregnations for degenerating axons, Cajal''s gold chloride-sublimate for astrocytes and Penfield''s modified silver carbonate for oligodendroglia and microglia. These, as well as Nissl and myelin sheath preparations show minimal shrinkage and distortion.