Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis
Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis
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DOI：
10.1128/iai.72.6.3658-3663.2004
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发表时间：
2004-06-01
影响因子：
3.1
通讯作者：
Murray, BE
中科院分区：
文献类型：
--
作者：
Mohamed, JA;Huang, W;Murray, BE
粪肠球菌心内膜炎分离株比非心内膜炎分离株更常产生生物膜，79株分离株中的39%比84株分离株中的6%产生强生物膜（P < 0.0001）。esp不是必需的，但其存在与较高量的生物膜相关（P < 0.001）。dltA、efaA、ace、lsa和6个双组分调节系统中被破坏的突变体在很大程度上没有改变，而epa、atn、gelE和fir中的破坏导致更少的附着细菌，如使用相差显微镜所确定的，以及更少的生物膜（P < 0.0001）。
Endocarditis isolates of Enterococcus faecalis produced biofilm significantly more often than nonendocarditis isolates, and 39% of 79 versus 6% of 84 isolates produced strong biofilm (P < 0.0001). esp was not required, but its presence was associated with higher amounts of biofilm (P < 0.001). Mutants disrupted in dltA, efaA, ace, lsa, and six two-component regulatory systems were largely unaltered, while disruptions in epa, atn, gelE, and fir resulted in fewer attached bacteria, as determined using phase-contrast microscopy, and less biofilm (P < 0.0001).