IMMUNOHISTOCHEMICAL STAINING OF HA-RAS ONCOGENE PRODUCT IN NORMAL, BENIGN, AND MALIGNANT HUMAN PANCREATIC TISSUES
IMMUNOHISTOCHEMICAL STAINING OF HA-RAS ONCOGENE PRODUCT IN NORMAL, BENIGN, AND MALIGNANT HUMAN PANCREATIC TISSUES
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DOI：
10.1016/s0046-8177(96)90006-4
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发表时间：
1990-06-01
期刊：
影响因子：
3.3
通讯作者：
ITOH, H
中科院分区：
文献类型：
--
作者：
SHIMIZU, M;SAITOH, Y;ITOH, H
我们检测了Ha-ras癌基因产物在正常、良性和恶性胰腺组织中的免疫组织化学染色。在胰腺癌病例中，评估其与组织学类型的关系。浆液性囊腺瘤和不典型腺泡细胞结节不与Ha-ras癌基因产物反应，正常胰腺导管细胞和腺泡细胞的免疫反应性低于其他良、恶性病变。而在正常胰腺组织中，胰岛细胞的阳性率与胰腺癌患者几乎相同。慢性胰腺炎阳性率最高，胰岛细胞瘤阳性率也较高。胰腺癌中，高分化腺癌的阳性率明显高于低分化腺癌。我们的研究表明，Ha-ras癌基因p21产物与人胰腺的肿瘤转化无关，不是鉴别胰腺良恶性病变的有用标记物，也不能用于确定胰腺分化的来源。
We examined immunohistochemical staining with Ha-ras oncogene product in normal, benign, and malignant human pancreatic tissues. In cases of pancreatic cancer, its relation to histologic type was evaluated. Serous cystadenoma and atypical acinar cell nodules did not react with the Ha-ras oncogene product, and ductal cells and acinar cells in normal pancreas showed lower immunoreactivity than other benign or malignant lesions. However, the positive rate of islet cells in normal pancreas was almost the same in cases of pancreatic cancer. Strongest positivity was observed in cases of chronic pancreatitis, and islet cell tumors also showed high positive staining rates. In pancreatic cancer, the positive rate of well-differentiated adenocarcinomas was rather higher than that of poorly differentiated adenocarcinomas. Our study indicates that the Ha-ras oncogene p21 product does not correlate with neoplastic transformation in human pancreas, is not a useful marker for differentiating benign and malignant lesions, and cannot be used to determine the origin for differentiation in the pancreas.