Contribution of anaphase B to chromosome separation in higher plant cells estimated by image processing
Contribution of anaphase B to chromosome separation in higher plant cells estimated by image processing
复制标题
DOI：
10.1093/pcp/pcm117
复制
发表时间：
2007-10-01
影响因子：
4.9
通讯作者：
Hasezawa, Seiichiro
中科院分区：
文献类型：
--
作者：
Hayashi, Tomomi;Sano, Toshio;Hasezawa, Seiichiro
后期可分为后期A和后期B两个亚期，但后期B在高等植物细胞中还没有明确的描述。在这项研究中，我们的时间顺序跟踪烟草BY-2细胞的染色体分离和纺锤体伸长的动态，分别使用组织荧光蛋白（RFP）和绿色荧光蛋白（GFP）微管蛋白。用图像处理技术对染色体和纺锤体边缘的位置进行了测定，结果表明，后期B对染色体分离距离的贡献约为40，与动物细胞中的染色体分离距离相当。这些结果表明高等植物细胞可能具有后期B过程。
Anaphase can be categorized into the two subphases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histonered fluorescent protein (RFP) and green fluorescent protein (GFP)tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed that anaphase B contributed to about 40 of the chromosome separation in distance, which is comparable with that in animal cells. These results suggest that higher plant cells potentially possess the process of anaphase B.