The effect of total starvation and very low energy diet in lean men on kinetics of whole body protein and five hepatic secretory proteins
The effect of total starvation and very low energy diet in lean men on kinetics of whole body protein and five hepatic secretory proteins
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DOI：
10.1152/ajpendo.00169.2007
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发表时间：
2007-12-01
影响因子：
5.1
通讯作者：
Elia, Marinos
中科院分区：
文献类型：
--
作者：
Afolabi, Paul R.;Jahoor, Farook;Elia, Marinos
Afolabi PR, Jahoor F, Jackson AA, Stubbs J, Johnstone AM, Faber P, Lobley G, Gibney E, Elia M.全饥饿和极低能量饮食对瘦男性全身蛋白质和5种肝脏分泌蛋白动力学的影响。[J] .中国生物医学工程学报，2009,31(2):559 - 559。首次发表于2007年9月18日；doi: 10.1152 / ajpendo.00169.2007。-目前尚不清楚体重减轻的速度是否会影响全身蛋白质代谢以及特定肝脏分泌蛋白的合成和血浆浓度。我们研究了1)瘦男性体重迅速减轻（饥饿）是否比那些体重减轻较慢的人在全身蛋白质动力学、合成和选定的肝脏分泌蛋白循环浓度方面表现出更大的变化[极低能量饮食（VLED）]；2)这些蛋白的血浆浓度与合成速率是否相关。采用[1-C-13]亮氨酸测定了11名瘦人（6名饥饿，5名VLED）的全身蛋白质动力学。采用定常静脉输注[C-13(2)]甘氨酸，测定hdl -载脂蛋白A1 （apoA1）、视黄醇结合蛋白、转甲状腺素、α 1-抗胰蛋白酶（α 1-AT）和转铁蛋白的部分合成率和绝对合成率。与VLED组相比，饥饿组全身蛋白质氧化水平显著升高（体重减轻5%）（P < 0.05）；HDL-apoA1合成率（25.3比-1.52%,P = 0.003）和视黄醇结合蛋白合成率（30.6比7.1%,P = 0.007）；HDL-apoA1的绝对合成率（7.1 vs. -3.8 mg.kg(-1).day）；P - 0.003)和α - 1-AT (17.8 vs. 3.6 mg.kg(-1).day)；P = 0.02)；血浆α 1-AT浓度（P = 0.025）。合成率和血浆浓度之间的关系因分泌蛋白而异。由此可见，瘦人肝脏分泌蛋白的合成速率与减肥速率的关系比与减肥幅度的关系更为密切。这些分泌蛋白的浓度变化可以独立于合成速率的变化而发生，反之亦然。
Afolabi PR, Jahoor F, Jackson AA, Stubbs J, Johnstone AM, Faber P, Lobley G, Gibney E, Elia M. The effect of total starvation and very low energy diet in lean men on kinetics of whole body protein and five hepatic secretory proteins. Am J Physiol Endocrinol Metab 293: E1580-E1589, 2007. First published September 18, 2007; doi: 10.1152/ajpendo.00169.2007. - It is unclear whether the rate of weight loss, independent of magnitude, affects whole body protein metabolism and the synthesis and plasma concentrations of specific hepatic secretory proteins. We examined 1) whether lean men losing weight rapidly ( starvation) show greater changes in whole body protein kinetics, synthesis, and circulating concentrations of selected hepatic secretory proteins than those losing the same amount of weight more slowly [ very low energy diet (VLED)]; and 2) whether plasma concentrations and synthetic rates of these proteins are related. Whole body protein kinetics were measured using [1-C-13] leucine in 11 lean men ( 6 starvation, 5 VLED). Fractional and absolute synthetic rates of HDL-apolipoprotein A1 (apoA1), retinol binding protein, transthyretin, alpha 1-antitrypsin (alpha 1-AT), and transferrin were measured using a prime-constant intravenous infusion of [C-13(2)] glycine. Compared with VLED group, the starvation group showed greater increases ( at a 5% weight loss) in whole body protein oxidation ( P < 0.05); fractional synthetic rates of HDL-apoA1 (25.3 vs. -1.52%; P = 0.003) and retinol binding protein (30.6 vs. 7.1%; P = 0.007); absolute synthetic rates of HDL-apoA1 (7.1 vs. -3.8 mg.kg(-1).day(-1); P - 0.003) and alpha 1-AT (17.8 vs. 3.6 mg.kg(-1).day(-1); P = 0.02); and plasma concentration of alpha 1-AT ( P = 0.025). Relationships between synthetic rates and plasma concentrations varied between the secreted proteins. It is concluded that synthetic rates of hepatic secreted proteins in lean men are more closely related to the rate than the magnitude of weight loss. Changes in concentration of these secreted proteins can occur independently of changes in synthetic rates, and vice versa.