Nerve Growth Factor Promotes Differentiation of Odontoblast-Like Cells
Nerve Growth Factor Promotes Differentiation of Odontoblast-Like Cells
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DOI：
10.1002/jcb.22006
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发表时间：
2009-03-01
影响因子：
4
通讯作者：
Sugiyama, Toshihiro
中科院分区：
文献类型：
--
作者：
Arany, Szilvia;Koyota, Souichi;Sugiyama, Toshihiro
当代的牙齿修复策略明显依赖于牙齿发育的细胞和生物成分的最新发现。在已知的几种生物活性分子中，神经营养素最近被认为可以影响牙胚细胞的增殖、分化和细胞-细胞外基质的相互作用。本研究旨在探讨神经生长因子(NGF)对牙乳头间充质细胞系永生化的影响。NGF在体外诱导成牙本质细胞系细胞分化，随后发生生物矿化。在这里，我们发现组织特异性标志物如DSPP和DMP I的标准化转录水平显著升高，表明细胞分化和成熟过程。我们进行了创新的基因表达分析，即TM14，一种基质细胞蛋白，也是纤维蛋白家族的新成员。TM14的表达模式与DMP-1相似，提示其在牙本质钙化过程中的细胞-基质和细胞间相互作用中起重要作用。碱性磷酸酶检测证实神经生长因子组有细胞外基质钙化。因此，NGF被认为是牙本质形成过程中矿化的有力促进因素。我们首次将TM14纳入成牙本质细胞的基因分型，并证明NGF在体外也能促进成牙本质细胞的分化。总之，这些结果强调了NGF在牙齿形态发生中的重要性，并促使复杂的上皮-间充质组织培养的精化，在那里可以完成对信号因子网络的进一步阐明。J.细胞。生物化学。106：539-545,2009。(C)2009年Wiley-Liss，Inc.
Contemporary strategies in tooth repair markedly rely on the newest findings on the cellular and biological components of dental development. Among several identified bioactive molecules, neurotrophins were recently proposed to affect tooth germ cell proliferation, differentiation, and cell-extracellular matrix interactions. The present study attempted to explore the effect of nerve growth factor (NGF) on a spontaneously immortalized dental papilla mesenchymal cell line. NGF induced differentiation of odontoblast-lineage cells with subsequent biomineralization in vitro. Here we showed that normalized transcript levels of tissue-specific markers such as DSPP and DMP I were elevated significantly, indicating cell differentiation and maturation processes. We performed innovative gene expression analysis or TM14, a matricellular protein and novel member of the fibulin family. TM14 expression followed a pattern similar to that of DMP 1, which suggests its important role in cell-matrix and intercellular interactions during dentin calcification. Alkaline phosphatase enzyme assay confirmed the extracellular matrix calcifications in NGF-supplemented groups. Thus, NGF was characterized as a potent promoter of mineralization during dentin formation. For the first time, we included TM14 in odontoblast genotype analysis and proved that NGF also promotes in vitro odontoblast differentiation. Collectively, these results highlight the importance of NGF during tooth morphogenesis, as well as urge the elaboration of complex epithelial-mesenchymal tissue cultures, where further elucidation of the signaling factor network could be completed. J. Cell. Biochem. 106: 539-545, 2009. (C) 2009 Wiley-Liss, Inc.