Periodontal therapy alters gene expression of peripheral blood monocytes
Periodontal therapy alters gene expression of peripheral blood monocytes
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DOI：
10.1111/j.1600-051x.2007.01113.x
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发表时间：
2007-09-01
影响因子：
6.7
通讯作者：
Pavlidis, Paul
中科院分区：
文献类型：
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作者：
Papapanou, Panos N.;Sedaghatfar, Michael H.;Pavlidis, Paul
目的：我们研究了牙周治疗对外周血单核细胞基因表达的影响。方法：15例牙周炎患者在4个时间点采集血样：牙周治疗前1周（#1），治疗开始时（基线，#2），6周（#3）和基线后10周（#4）。在基线和10周时，记录牙周状况，并获得龈下菌斑样本。牙周治疗（牙周手术和拔牙，不使用抗生素）在6周内完成。在每个时间点，测定19种生物标志物的血清浓度。纯化外周血单核细胞，提取RNA，反转录，标记并与AffymetrixU133Plus2.0芯片杂交。使用线性随机效应模型分析表达谱。基因本体论术语的进一步分析将表达模式归纳为生物学相关类别。所选基因的差异表达证实了实时逆转录-聚合酶链反应在一个子集的patients.Results：治疗导致了临床牙周状况的大幅改善和几个牙周病原体的水平降低。随时间的表达谱显示超过11，000个探针组以< 0.05的错误发现率差异表达。大约三分之一的患者表现出与先天免疫、细胞凋亡和细胞信号传导相关的基因表达的显着变化。结论：数据表明，牙周治疗可能会改变单核细胞基因表达，其方式与全身抗炎作用一致。
Aims: We investigated the effects of periodontal therapy on gene expression of peripheral blood monocytes.Methods: Fifteen patients with periodontitis gave blood samples at four time points: 1 week before periodontal treatment (#1), at treatment initiation (baseline, #2), 6-week (#3) and 10-week post-baseline (#4). At baseline and 10 weeks, periodontal status was recorded and subgingival plaque samples were obtained. Periodontal therapy (periodontal surgery and extractions without adjunctive antibiotics) was completed within 6 weeks. At each time point, serum concentrations of 19 biomarkers were determined. Peripheral blood monocytes were purified, RNA was extracted, reverse-transcribed, labelled and hybridized with AffymetrixU133Plus2.0 chips. Expression profiles were analysed using linear random-effects models. Further analysis of gene ontology terms summarized the expression patterns into biologically relevant categories. Differential expression of selected genes was confirmed by real-time reverse transcriptase-polymerase chain reaction in a subset of patients.Results: Treatment resulted in a substantial improvement in clinical periodontal status and reduction in the levels of several periodontal pathogens. Expression profiling over time revealed more than 11,000 probe sets differentially expressed at a false discovery rate of < 0.05. Approximately 1/3 of the patients showed substantial changes in expression in genes relevant to innate immunity, apoptosis and cell signalling.Conclusions: The data suggest that periodontal therapy may alter monocytic gene expression in a manner consistent with a systemic anti-inflammatory effect.