Fingerprint profile of Ginkgo biloba nutritional supplements by LC/ESI-MS/MS
Fingerprint profile of Ginkgo biloba nutritional supplements by LC/ESI-MS/MS
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DOI：
10.1016/j.phytochem.2008.01.026
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发表时间：
2008-05-01
期刊：
影响因子：
3.8
通讯作者：
Brenton, A. G.
中科院分区：
文献类型：
--
作者：
Ding, S.;Dudley, E.;Brenton, A. G.
银杏是最受欢迎的草药营养补充剂之一，萜内酯和黄酮类化合物是两种主要的活性成分。采用毛细管高效液相色谱/质谱联用技术，建立了一种可鉴定出70多个组分的指纹图谱方法。biloba产品该方法允许同时检测黄酮类化合物和萜类内酯，并且只需一次HPLC/MS运行即可获得母离子及其碎片的信息。有针对性的采集后分析允许关于类黄酮组分的鉴定的质谱信息容易地与其他数据区分开，然而，由于二聚体形成，萜烯内酯的相同方法不太成功，需要进一步开发。对5种商品G.比较了银杏营养强化剂的营养成分，观察了样品间某些成分的变化，并进行了强化检测。在对G.银杏产品这种方法可以被看作是补充具体的定量分析的一些生物活性成分的草药。（C）2008爱思唯尔有限公司保留所有权利。
Ginkgo biloba is one of the most popular herb nutrition supplements, with terpene lactones and flavonoids being the two major active components. A fingerprint profile method was developed using a capillary HPLC/MS method which can identify more than 70 components from the G. biloba product. The method allows the flavonoids and terpene lactones to be detected simultaneously and information of both the parent ion and its fragmentation can be obtained in just one HPLC/MS run. Targeted post-acquisition analysis allows mass spectrometric information regarding the identification of flavonoid components to be easily distinguished from other data, however the same approach for terpene lactones was less successful due to dimer formation and requires further development. The fingerprint profiles of five commercial G. biloba nutritional supplements were obtained and compared; variation of some components among the samples was observed and fortification could be detected. In the quality control analysis of the G. biloba product this method could be viewed as complementary to specific quantitative analysis of some bioactive components of the herb. (C) 2008 Elsevier Ltd. All rights reserved.